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Validated method to determine (±)-gossypol in Candida tropicalis culture by high-performance liquid chromatography

Cheng C., Cun-Xi N., Jing L., Yong-Qiang W., Yan-Feng L., Wen-Xia G., Wen-Ju Z.

Acta Chromatographica

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2018

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Vol. 30, no. 4

269--273

EN

A validated high-performance liquid chromatography (HPLC) method has been developed to analyze the (±)-gossypol in the selection of strains of Candida tropicalis culture. Since gossypol was easily degraded and oxidized, the addition of antioxidant NADPH-Na4 and acetone extraction was chosen to prevent gossypol degradation and gradient elution assay was applied to obtain gossypol resolution. Concentrations of gossypol in C. tropicalis ZD-3 culture 20 μg/mL were determined, and concentration–time profiles were observed. Linearity of the gossypol standard curve by HPLC area method was ranged from 0.1 to 20 μg/mL with Y = 26.954 × X − 29.547, R2 = 0.9991, and n = 3, with limit of detection (LOD) of 50 ng/mL and lower limit of quantification (LLOQ) of 500 ng/mL. The recovery rate is dose-dependent and ranged from 85.3% to 103.5%. It is a rapid and reliable HPLC method for gossypol quantization in microorganism culture which could be applied in solid fermentation in the feed industry.

2

Excretion study of 10-Methoxycamptothecin and its metabolite 10-Hydroxycamptothecin in rats by RP-HPLC method with fluorescence detection

Changmin S., Zheng J., Chi J., Jing L., Xiufeng Y., Wang Y.

Acta Chromatographica

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2017

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Vol. 29, no. 4

453--458

EN

10-Methoxycamptothecin (MCPT) and 10-hydroxycamptothecin (HCPT) are the indole alkaloids isolated from a Chinese tree, Camptotheca acuminata, and have a wide spectrum of anticancer activity in vitro and in vivo mainly through inhibitory effects on topoisomerase I. HCPT is a major metabolite of MCPT in rats; the pharmacokinetic analysis and tissue distribution of MCPT and HCPT in rats have also been determined after i.v. injection of MCPT, but the excretion of MCPT and its metabolite HCPT has not been assessed up to now. In the present study, the excretion study of MCPT and its metabolite HCPT in rat bile, feces, and urine after i.v. administration of MCPT (5 mg kg-1) was performed by high performance liquid chromatography (HPLC) method coupled with a fluorescence detector. The results showed that MCPT mainly biotransformed to HCPT and excreted in the form of HCPT and MCPT in bile, urine, and feces after i.v. administration of MCPT. It was excreted about 1.24 ± 0.07% as MCPT and 5.49 ± 0.40% as HCPT in bile within 6 h after i.v. administration. The cumulative excretions of MCPT and HCPT were mainly within 24 h after i.v. administration, which were 0.41 ± 0.10% and 7.66 ± 1.43% of the dosage in urine and about 0.16 ± 0.04% and 20.30 ± 3.35% of the dosage in feces. The total excretion of MCPT in urine, bile, and feces was 1.81 ± 0.09% in the form of original MCPT and 33.45 ± 1.57%. detected as the metabolite HCPT in urine, bile, and feces, suggesting that MCPT might undergo other biotransformation.

3

Study on the Properties of Core Spun Yarn and Fabrics of Shape Memory Polyurethane

Jing L., Hu J.

Fibres & Textiles in Eastern Europe

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2010

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Vol. 18, no. 4 (81)

39--42

EN

Core spun yarn with shape memory fibre and cotton were developed using a ring spinning frame and friction spinning machine. Shape memory fabrics were woven from two kinds of shape memory core spun yarns, and the mechanical and shape memory properties of the yarns and fabrics were examined. The yarns have a good weave ability, and the yarns and fabrics have a good shape memory fix and recovery properties. This paper also introduces the application of shape memory yarns and fabrics in protective textiles and compares the shape memory properties of two kinds of shape memory core yarn and woven fabrics weft wise and warp wise.

PL

Opracowano przędze rdzeniowe z udziałem włókien poliuretanowych z pamięcią kształtu i bawełny, z wykorzystaniem przędzarki obrączkowej i frykcyjnej. Stosowano dwa rodzaje przędz rdzeniowych, następnie badano właściwości mechaniczne i pamięć kształtu przędz oraz wykonanych z nich tkanin. Wspomniane przędze nie nastręczają trudności w tkaniu i pozwalają na uzyskanie tkanin o dobrej pamięci kształtu. Artykuł przedstawia zastosowania tkanin i przędz z pamięcią kształtu jako materiałów ochronnych oraz porównuje właściwości dwóch rodzajów przędzy rdzeniowej z pamięcią kształtu i właściwości tkanin w kierunku osnowy i wątku.