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1 2018

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Catechin-molecularly imprinted cryogel for determination of catechin in red wines by HPLC–DAD–fluorescence detector

Büyüktuncel E., Porgalı E., Özkara S.

Acta Chromatographica

2018

Vol. 30, no. 1

54--61

A new molecularly imprinted polymer (MIP) was prepared by using catechin (C) as the template molecule. The polymer was characterized by swelling tests, scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET), and Fourier transform infrared (FTIR) spectroscopy. The MIP with high recovery was selected as a solid-phase extraction (SPE) sorbent in this work. The standard solutions were directly applied onto the SPE cartridges following loading, washing, and elution procedures. A solution of the collected fractions was analyzed by high-performance liquid chromatography–diode-array detection (DAD) and fluorescence detector. The optimization of the method and validation was achieved on a C18 column (5 μm, 250 × 4.6 mm) with methanol–water (35:65, v/v) mixture adjusted pH 2.5 as the mobile phase at a flow rate of 1 mL min−1 at room temperature. The selectivity coefficient (k) of imprinted p(HEMA–MAH) cryogel was 5.1-fold that of non-imprinted cryogel. It showed good selectivity and affinity for C molecule. A comparison was made between the results obtained with the MIP cartridges and a traditional C18 reversed-phase cartridge. It was observed that 2.3 times higher recovery of C can be obtained on catechin-MIP cryogel. The results of the presented work showed that the prepared MIP can be used as SPE sorbent for extracting of C from red wines.