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Quantification of piperine and 18-β glycyrrhetinic acid in herbal formulation “Eladi Gutika”

Rode S., Bhujbal P., Sandhya P.

Acta Chromatographica

2013

Vol. 25, no. 1

135--146

Eladi Gutika is a polyherbal formulation official in “Ayurvedic formulary of India” and used for dry cough and throat infection. A simple, specific and precise high-performance thin-layer chromatography (HPTLC) method has been developed for quantification of piperine and 18-β glycyrrhetinic acid in Eladi Gutika. We report the extraction and estimation of these compounds in a laboratory prepared sample of Eladi Gutika and two of its marketed formulations. The compounds were chromatographed on precoated silica gel G 60254 plates in the mobile phase comprising of toluene, ethyl acetate, and glacial acetic acid. Under the optimized chromatographic conditions, the calibration plot was found to be linear in the range of 0.2–1 g mL-1 with a correlation coefficient R2 = 0.9902 for piperine and 0.9904 for 18-β glycyrrhetinic acid. Mean recovery for piperine was 99.75% w/w and for 18-β glycyrrhetinic acid was 101.36% w/w.