To intensify biogas production during anaerobic stabilization of organic matter in sludge, pretreatment is applied. The effect of pretreatment of excess activated sludge (AS) and excess aerobic granular sludge (GS) on biogas productivity (BP) and composition was investigated. The sludge was pretreated with homogenization (6,500 rpm for 0.5 min (H0.5) and 1 min (H1.0)) or ultrasound disintegration at 20 kHz (50% amplitude for 2 min (D50%_2.0) and 4 min (D50%_4.0), and 100% amplitude for 4 min (D100%_4.0)). BP of AS of GS without pretreatment was 603.3±5 dm3/kg TS (793.4±7 dm3/kg VS); that was 200.6±4 dm3/kg TS (480.8±6 dm3/kg VS). With disintegration, the BP of AS increased by 7.8% (650.4±10 dm3/kg TS) (D50%_2.0) and 16.1% (700.6±11 dm3/kg TS) (D100%_4.0), and that of GS increased by 7.0% (214.0±5 dm3/kg TS) (D50%_2.0) and 16.0% (232.8±5 dm3/kg TS) (D100%_4.0). With homogenization, BP increased by 2.0-3.0% (AS) and 1.6-3.2% (GS).
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