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Biodentine management and setting time with Vicat and Vickers evaluation; a survey-based study on clinicians' experience

Buła K., Palatyńska-Ulatowska A., Klimek L.

Archives of Materials Science and Engineering

2020

Vol. 103, nr 2

75--85

Purpose: of this paper was to analyse clinicians’ views on the management and handling procedures of the Biodentine tricalcium silicate cement with the following evaluation of the real setting time of the material with two independent physical tests. Design/methodology/approach: A survey study included 174 clinicians who answered the questionnaire designed to collect opinions on the Biodentine management during endodontic procedures. To verify the setting time of the cement, two independent hardness tests were performed. Macroscopic evaluation was carried out using the Vicat device. Microscopic assessment with subsequent SEM observation was conducted with the aid of the Clemex appliance. Findings: 43% of respondents using Biodentine in their practice described the setting time as long or definitively too long. One fifth of the dentists surveyed continue dental procedures without waiting. The setting time tests confirmed the existence of two phases of the Biodentine setting process, which corresponds to the general definition of cement setting. After mixing of the material, the initial setting stage lasts for 15 minutes. The next one, described by the authors as “maturation” of Biodentine lasts for 120 minutes. Research limitations/implications: The material initially sets within 15 minutes, however it is not the end of the process. In certain endodontic procedures the awareness of a longer setting time of Biodentine is essential for decision-making in root canal therapy. Practical implications: It is advisable to divide the endodontic treatment with Biodentine into two separate appointments. Originality/value: From the clinicians’ perspective the setting time and correct handling of Biodentine are crucial factors in the successful endodontic therapy. The information regarding proper material management is included in this paper

Badania SEM, EDS oraz dyfrakcji rentgenowskiej materiału mineral trioxide aggregate w symulowanych warunkach klinicznych

Palatyńska-Ulatowska A., Klimek L.

Engineering of Biomaterials

2009

Vol. 12, no. 85

13--18

Mineral trioxide aggregate (MTA) jest materiałem, którego zastosowanie wywołuje odpowiedź biologiczną polegającą na regeneracji przyczepu łączno-tkankowego tkanek przyzębia oraz odbudowie kości. Jednakże hipoteza wpływu MTA na procesy gojenia i odbudowy tkanek okołozębowych oraz mechanizmu tworzenia cementu korzeniowego pozostaje niejasna. Celem pracy było zbadanie wpływu płynu tkankowego SBF (Simulated Body Fluid) na materiał Pro Root MTA Grey w obecności twardych tkanek zęba. Z 10 zębów bydlęcych uzyskano 20 bloczków cementu korzeniowego. Po opracowaniu próbek i nałożeniu preparatu MTA Grey podzielono je na trzy równe grupy (I, II i III), po 6 próbek każda. Bloczki zębiny z grupy I zanurzono w 0,9% roztworze soli fizjologicznej (0,9% NaCl), bloczki z grupy II i III inkubowano w syntetycznym płynie tkankowym (roztwór SBF) odpowiednio przez 2 i 10 miesięcy. Wszystkie próbki umieszczono w temp. 37oC oraz 100% wilgotności. Po okresie inkubacji próbki poddano badaniu w elektronowym mikroskopie skaningowym, wykonano analizę spektroskopową EDS oraz badanie metodą dyfrakcji rentgenowskiej. Na powierzchniach próbek inkubowanych w SBF stwierdzono obecność związków fosforu i wapnia, jednak nie stwierdzono obecności kryształków hydroksyapatytu.

Mineral trioxide aggregate (MTA) is a material provoking a good biological reaction which may facilitate the regeneration of the periodontal ligament and formation of bone. However, the hypothesis of the dental alveolar healing and cement formation by the application of MTA has not been proven. The purpose of this study was to investigate the probable influence of the Simulated Body Fluid (SBF) on Grey Pro Root Mineral Trioxide Aggregate (GMTA) in the presence of the dental hard tissue. From 10 bovine extracted teeth, 20 samples of hard dental tissue were obtained. After the preparation and application of the GMTA material all of the samples were divided randomly into three groups (I, II, III), of 6 samples each. Dentine blocks in group I were incubated in 0.9% NaCl whilst those in groups II and III were incubated in SBF for 2 and 10 months, respectively, at 37oC, 100% humidity. SEM investigation, energy dispersive spectroscopy elemental analysis (EDS) and X-ray diffraction analysis of the samples were performed. On the surfaces of SBF incubated samples precipitation of phosphorus and calcium was indicated but no hydroxyapatite crystals were found.