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Determination of stearic acid and 12-hydroxyoctadecanoic acid in PEG-60 hydrogenated castor oil by HPLC-ELSD after alkaline hydrolysis

Duan Yu, Liu Xian-Zhao, Li En-Nian, Guo Zhong-Jing, Xu Xin-Jun

Acta Chromatographica

2024

Vol. 36, no. 2

150--155

A rapid and simple method for the determination of stearic acid and 12-hydroxystearic acid in PEG-60 hydrogenated castor oil by high performance liquid chromatography with evaporative light scattering detection was established. The oil sample was first pretreated by alkaline hydrolysis. The analysis was performed on a Zhongpu Develop XD-C18 column (250mm 3 4.6 mm, 5 μm) with gradient elution of methanol and 1% acetic acid aqueous solution at a flow rate of 1.2 mL$min1 and a column temperature of 40 8C. The drift tube temperature of the evaporative light scattering detection system was set at 40 8C, and the pressure of carrier gas (N2) was 337 kPa. The regression equation revealed a good linear relation (r 5 0.9993–0.9995) during the test ranges (119.1–1190.7 μg$mL1 for 12-hydroxystearic acid, 10.7–107.4 μg$mL1 for stearic acid). The detection limits of 12-hydroxystearic acid and stearic acid were 1.1 and 2.5 μg$mL1, the limits of quantitation were 3.2 and 7.4 μg$mL1, respectively. And the mean recoveries were 101.5 and 101.0%, the corresponding relative standard deviations (RSDs) were 2.1 and 2.8%, respectively. The RSDs corresponding to repeatability (n 5 6) were both less than 1.7% in terms of precision. As to the stability, the test results remained stable after 8 h at room temperature (RSDs were both less than 2.6%). The developed method showed high sensitivity, recovery, repeatability and stability, which indicated that the method could be applied as a quality evaluation method for the determination of stearic acid and 12-hydroxyoctadecanoic acid in PEG-60 hydrogenated castor oil.