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High-performance thin-layer chromatographic analysis of neutral lipids and phospholipids in Cerithidia californica (Gastropoda) infected with three species of larval trematodes

Cline D. J., Fried B., Sherma S.

Acta Chromatographica

2000

No. 10

183-189

High-performance thin-layer chromatography (HPTLC) has been used to analyze neutral lipids and phospholipids in the digestive gland–gonad complex (DGG) of Cerithidia californica snails infected with three species of larval trematode: Euhaplorchis californiensis, Cloacitrema michiganensis, and Mesostephanus appendiculatus. C. californica not infected with larval trematodes were used as control snails. The triacylglycerol content of the DGGs of snails infected with the three species of larval trematodes was significantly less than that of the controls. There was no significant difference between the free sterol content of DGGs infected with any larval trematode type compared with controls. The cholesteryl ester content was significantly lower than that of the controls only in snails infected with C. michiganensis. The situation was variable for free fatty acid content, with M. appendiculatus and E. califor-niensis infections resulting in a significant increase in the concentration of this fraction, compared with that of the controls, and C. michiganensis resulting in a significant decrease. Phosphatidylethanolamine content was increased significantly by all three infections and phosphatidylcholine content was increased by a factor of two or three compared with controls. In conclusion, larval parasitism in C. californica induces different lipid profiles in this marine snail, and these differences can provide chemo-taxonomic information for discrimination between larval trematodes from the snail host.

TLC and GC-MS identification of glucose and maltose in Biomphalaria glabrata (Gastropoda), and use of quantitative TLC to determine the effect of starvation on the amounts of these carbohydrates

Cline D. J., Fried B., Sherma J.

Acta Chromatographica

1999

No. 9

79-86

Maltose and glucose have been identified as the primary carbohydrates in the planorbid snail Biomphalaria glabrata by high performance thin layer chromatography (HPTLC) on silica gel, amino-bonded, C18 reversed-phase, and cellulose plates. The use of specific detection reagents for reducing sugars, spiking experiments, and differential coloration of sugar zones with ?-naphthol reagent on amino plates aided the identification of glucose and maltose. Gas chromatography–mass spectrometry (GC–MS) analysis confirmed the identification maltose and glucose on the basis of retention times and spectral fingerprints. The presence of trehalose was not confirmed. After identifying maltose and glucose, snails were starved for twelve days and the effects on the concentrations of these sugars were determined. Amounts of glucose dropped significantly in the digestive gland–gonad complex (DGG) from day 0 to day 12; maltose in the hemolymph and DGG and glucose in the hemolymph did not change significantly during the starvation period.