Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Acta Chromatographica

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Rapid analysis of loratadine in human serum by high-performance liquid chromatography with fluorescence detection

Plenis A., Konieczna L., Olędzka I, Kowalski P.

Acta Chromatographica

2010

Vol. 22, no. 1

69--79

A simple and rapid high-performance liquid chromatographic method with fluorescence detection for analysis of loratadine (LOR) in small volumes of human serum has been developed and validated. After solid-phase extraction (SPE), with thioridazine hydrochloride as internal standard, chromatographic separation was performed on a C 18 analytical column with 70:30 ( v / v ) acetonitrile-water, adjusted to pH 2.7 with orthophosphoric acid as mobile phase at a flow-rate of 1 min mL -1. The column was maintained at 28°C. Fluorescence detection was performed at excitation and emission wavelengths of 265 of 454 nm, respectively. The method was validated for accuracy, precision, selectivity, linearity, recovery, and stability. Absolute recovery of LOR was >93.0%. The limits of detection (LOD) and quantification (LOQ) were 0.07 and 0.2 ng mL -1,respectively. Linearity was confirmed in the range 0.2–30 ng mL -1 (correlation coefficient >0.9998). This HPLC method is selective, robust, and specific and would enable efficient analysis of large numbers of serum samples in support of pharmacokinetic, bioavailability, or bioequivalence studies after therapeutic doses of LOR.

Quantitative determination of pentoxifylline in human plasma

Chmielewska A., Konieczna L., Plenis A., Lamparczyk H.

Acta Chromatographica

2006

No. 16

70-79

A rapid, optimized, and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for deter-mination of pentoxifylline in human plasma. The analyte was extracted from the plasma with dichloromethane after addition of 0.2 mL 1 M NaOH. HPLC separation was performed on a C18 analytical column (250 mm × 4 mm i.d.) with acetonitrile–water, 45:55 (v/v), as mobile phase. Spectro-photometric detection was performed at 275 nm. Calibration graphs were linear from 25 to 1000 ng mL-1 pentoxifylline. Recovery of the drug from human plasma was 92.1%, and the detection limit was 20 ng mL-1.