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1 Polish Journal of Chemistry

1 1999

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Inhibition of jack bean urease by a mixture of boric acid and phosphate buffer pH 6.96

Krajewska B., Zaborska W., Leszko M., Brzózka Z.

Polish Journal of Chemistry

1999

vol. 73 nr 2

359-366

Inhibition of jack bean urease by a mixture of boric acid and phosphate buffer pH 6.96 was studied. The inhibition constants of the mixture were determinated in solutions of phosphate buffer (22-155mM) by measuring the initial reaction rates for a series of boric acid concentrations (0-3 mM) at a series of substrate concentrations (2-50 mM urea). It was found that boric acid is a simple competitive inhibitor of urea se with the inhibition constant of the system changing from 0.23 mM in 22 mM phosphate buffer to 0.76 mM in 155 mM phosphate buffer. It was shown, that boric acid and phosphate buffer pH 6.96 are mutually exclusive competitive inhibitors of jack bean urease. The inhibition constants of boric acid and of phosphate buffer pH 6.96 in an inhibitor-free system were determinated to be 0.12 mM and 21 mM, respectively.