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1

A novel approach for detection and delineation of cell nuclei using feature similarity index measure

John J., Nair M. S., Kumar P. R. A., Wilscy M.

Biocybernetics and Biomedical Engineering

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2016

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Vol. 36, no. 1

76--88

EN

Accurate image segmentation of cells and tissues is a challenging research area due to its vast applications in medical diagnosis. Seed detection is the basic and most essential step for the automated segmentation of microscopic images. This paper presents a robust, accurate and novel method for detecting cell nuclei which can be efficiently used for cell segmentation. We propose a template matching method using a feature similarity index measure (FSIM) for detecting nuclei positions in the image which can be further used as seeds for segmentation tasks. Initially, a Fuzzy C-Means clustering algorithm is applied on the image for separating the foreground region containing the individual and clustered nuclei regions. FSIM based template matching approach is then used for nuclei detection. FSIM makes use of low level texture features for comparisons and hence gives good results. The performance of the proposed method is evaluated on the gold standard dataset containing 36 images (_8000 nuclei) of tissue samples and also in vitro cultured cell images of Stromal Fibroblasts (5 images) and Human Macrophage cell line (4 images) using the statistical measures of Precision and Recall. The results are analyzed and compared with other state-of-the-art methods in the literature and software tools to prove its efficiency. Precision is found to be comparable and the Recall rate is found to exceed 92% for the gold standard dataset which shows considerable performance improvement over existing methods.

2

Nowelizacja przepisów normalizacyjnych dotyczących badań taboru kolejowego w zakresie EMC

Dłużniewski A., John J., Laskowski M.

TTS Technika Transportu Szynowego

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2015

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R. 22, nr 12

402--405, CD

PL

W artykule przedstawiono metodykę pomiarową emisji zaburzeń promieniowanych i przewodzonych w pokładowej sieci zasilającej niskiego napięcia taboru kolejowego na przykładzie obecnie obowiązujących wymagań normatywnych norm serii PN-EN 50121-x-x. Przedstawiono szczegółowo metodykę pomiarową, dopuszczalne po-ziomy emisji zaburzeń promieniowanych na postoju i w trakcie jazdy pojazdu oraz emisji zaburzeń przewodzonych w pokładowej sieci zasilającej niskiego napięcia. Na koniec dokonano porównania obowiązujących wymagań normatywnych w tym zakresie z nowymi edycjami norm kolejowych, które mają zostać wprowadzone niebawem, ze względu na dość stare wydania obecnych dokumentów normalizacyjnych, w oparciu które należy badać tabor kolejowym pod kątem EMC celem spełnienia wymagań normatywnych.

EN

The paper presents the methodology of measurement radiation and conducting disturbance emission in onboard rolling stock power low voltage network in reference to current obligatory normative standard requirements PN-EN 50121-x-x. The article presents in detail the methodology of measurement, methodology of permissible levels of radiation emission disturbance during stand and during vehicle ride and the levels of conducted disturbance emission in on-board low voltage power network. In conclusion paper presents com-parison of obligatory, normative standard requirements in this range and along with new edition of rail standards, which will be deploy soon, due to the old editions of current standards documents based on rolling stock should be tested in terms of EMC in order to fulfill normative requirements.

3

Characterization of solvents containing CyMe4-BTPhen in selected cyclohexanone-based diluents after irradiation by accelerated electrons

Distler P., Kondé J., John J., Hájková Z., Švehla J., Grüner B.

Nukleonika

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2015

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Vol. 60, No. 4, part 2

885--891

EN

Radiation stability of CyMe4-BTPhen was examined in systems with three selected cyclohexanone-based diluents. Accelerated electrons were used as a source of ionizing radiation. The CyMe4-BTPhen radiation degradation identifi cation and characterization of the degradation products were performed by high performance liquid chromatography (HPLC) and mass spectrometry (MS) analyses. Residual concentrations of tested ligand were determined. Moreover, extraction properties of the solvents irradiated at two different doses were compared with the extraction properties of non-irradiated solvents to estimate the influence of the presence of degradation products in the organic phase.

4

The upper edge geodetic number and the forcing edge geodetic number of a graph

Santhakumaran A. P., John J.

Opuscula Mathematica

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2009

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Vol. 29, no. 4

427-441

EN

An edge geodetic set of a connected graph G of order p ≥ 2 is a set S ⊆ V(G) such that every edge of G is contained in a geodesic joining some pair of vertices in S. The edge geodetic number g1(G) of G is the minimum cardinality of its edge geodetic sets and any edge geodetic set of cardinality g1(G) is a minimum edge geodetic set of G or an edge geodetic basis of G. An edge geodetic set S in a connected graph G is a minimal edge geodetic set if no proper subset of S is an edge geodetic set of G. The upper edge geodetic number g1+(G) of G is the maximum cardinality of a minimal edge geodetic set of G. The upper edge geodetic number of certain classes of graphs are determined. It is shown that for every two integers a and b such that 2 ≤ a ≤ b, there exists a connected graph G with g1(G) = a and g1+(G) = b. For an edge geodetic basis S of G, a subset T ⊆ S is called a forcing subset for S if S is the unique edge geodetic basis containing T. A forcing subset for S of minimum cardinality is a minimum forcing subset of S. The forcing edge geodetic number of S denoted by ƒ1(S), is the cardinality of a minimum forcing subset of S. The forcing edge geodetic number of G, denoted by ƒ1(G), is ƒ1(G) = min{ ƒ1(S)}, where the minimum is taken over all edge geodetic bases S in G. Some general properties satisfied by this concept are studied. The forcing edge geodetic number of certain classes of graphs are determined. It is shown that for every pair a, b of integers with 0 ≤ a < b and b ≥ 2, there exists a connected graph G such thatƒ1(G) = a and g1(G) = b.

5

Assessment of river health in Australia by diatom assemblages - a review

John J.

Oceanological and Hydrobiological Studies

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2004

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Vol. 33, No. 2

95--104

EN

This paper summarises the findings of two national projects carried out by the author, covering the largest area ever investigated on the use of diatoms for monitoring river health in Australia, as well as to recommend future directions in diatom river monitoring in Australia. The first project (1995 to 1998) involved South-west Western Australia – well known for its forests and abundance of streams and rivers. Data on environmental variables and diatom taxa were obtained from 136 sites, out of which 117 were used as reference sites and 29 as monitoring (impacted) sites. Some 20 sites were randomly selected from the reference sites and used as "test sites" to verify internal consistency of the reference sites which were considered to be relatively pristine. The streams and rivers were finally classified into four distinct groups based upon environmental factors and diatom distribution pattern. The second project involved monitoring the health of urban streams around the city of Perth, western Australia. The investigation (1996 to 1999) focussed on classification of the urban streams based on water quality parameters and "stream conditions" and development of a predictive model using diatoms as biomonitors. Close to 180 sites were sampled with 30 environmental variables measured. All the sites were classified on the basis of seven environmental variables with the highest correlation coefficient with the sites, using the multivariate pattern analysis program PATN. The reference and monitoring sites were mostly separated on the basis of environmental factors and distinct diatom assemblages. Currently, a national protocol for assessing the health of all rivers using diatoms in Australia is being compiled.

6

Reometryczne badania procesu sieciowania polietylenu i kopolimerów etylenu wobec nadtlenków. Cz. 1, Opracowanie metodyki badań

Uhniat M., Zemlak M., John J., Szulc R.

Polimery

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2003

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T. 48, nr 10

709-713

PL

Zbadano możliwość zastosowania do kontroli procesu sieciowania PE-LD powszechnie używanego w ocenie przebiegu wulkanizacji gumy reometru rotacyjnego "Wulkametr WG-03" produkcji Zakładów Automatyki Chemicznej Metal-chem w Gliwicach. Sporządzono trzy różniące się składem stabilizowane kompozycje PE-LD z nadtlenkiem dikumylu i poddano je sieciowaniu w wulkametrze w warunkach izotermicz-nych w temp. 170, 180 i 200°C. Na podstawie krzywych zależności momentu skręcającego (M) od czasu (t) oznaczono następujące parametry charakterystyczne procesu sieciowania: minimalną i maksymalną wartość M, czas inicjowania procesu sieciowania i czas, po którym M uzyskuje maksymalną wartość. Stwierdzono dużą powtarzalność zależności M = f(t) w stałej temperaturze. Względne odchylenia standardowe parametrów charakterystycznych procesu sieciowania PE-LD mieszczą się w przedziale od 2,5 do 7,1% wartości średniej wyznaczonej z 10 pomiarów. Otrzymane wyniki dowodzą, że "Wulkametr WG-03" (po jego małej modyfikacji) i opisana metoda mogą być stosowane do kontroli procesu sieciowania polietylenu, a więc w szerszym zakresie niż wynika to z pierwotnego przeznaczenia tego aparatu.

EN

Possibility of applying of a rheometer "Wulkametr WG-03" (produced by "Metalchem", Gliwice, Poland, Fig. 1), widely used for rubber curing course estimation, to checking of PE-LD crosslinking process was studied. Three various stabilized compositions of PE-LD and dicumyl peroxide (Table 1) were prepared and crosslinked in the vulcameter, at isothermal conditions at 170, 180 or 200degreesC. On the basis of curves of torque moment (M) versus time (t) dependences, the following characteristic parameters of crosslinking process (Fig. 2) have been determined: minimal and maximal M value, initiation time of crosslinking and time of maximal M. Very good repeatability of M = f(t) dependence at constant temperature (Fig. 3) has been found. Relative standard deviations of characteristic parameters of PE-LD crosslinking process are placed in the range from 2.5 to 7.1% of average values determined from 10 measures (Tables 2-4). The results obtained show that "Wulkametr WG-03" (after slight modification) and the method described can be used for polyethylene crosslinking process checking so wider than original purpose of the apparatus.

7

W kierunku zyskownych usług - ewolucja sieci następnej generacji

Streilein P., John J.

Przegląd Telekomunikacyjny + Wiadomości Telekomunikacyjne

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2001

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nr 12

849-852

PL

Omówiono ewolucję sieci transmisji danych w kierunku wytyczanym przez nowe rozwiązania, oparte na przełączaniu wielousługowym oraz inteligentnej sieci optycznej.

EN

The paper discusses the evolution of data networks towards new solutions, based on multiservice routing/switching and the emerging intelligent optical layer.

8

Lead chalcogenide on silicon infrared sensor arrays

Zogg H., John J.

Opto-Electronics Review

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1998

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Vol. 6, No. 1

37-46

EN

Narrow gap PB₁₋xSnxSe and PbTe layers are grown epitaxially on Si(111) - substrates by molecular beam epitaxy (MBE). They exhibit high qulity despite the large lattice and thermal expansion mismatch. Thermal strains relax without adversely affecting the structural quality of the layers even at cryogenic temperatures and after many temperature cyclings. This is due to easy glide of misfit dislocations in the layers. Photovoltaic infrared sensor arrays of the Schottky type with Pb blocking contacts are fabricated. Cut-off wavelengths range from 4 to 12 µm. Above 150 K, the noise currents tend to the values predicted by the Schottky theory.At lower temperatures, the noise currents are determined by the density of dislocations in the layers. E. g. for PbSe (7 um cut-off wavelength at 80 K) each threading dislocation in the active area gives rese to a shunt resistance of 1.2. G Ω. If the dislocation density is reduced to below 2 x 10⁶ cm⁻², the influence of the dislocations becomes negligible. Layers such low dislocation densities have been obtained by thermal cycling. Thermal imaging is demonstrated with linear arrays with 128 sensors either hybridly bonded to Si-read-out electronics, or with arrays fabricated in layers grown epitaxially onto Si-chips which already contain the read-out circuits.