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HPLC analysis and detection of l-deprenyl

Csomor V., Laufer R., Pöstényi Z., Kalász H., Adeghate E., Nurulain S. M., Tekes K., Adem A.

Acta Chromatographica

2014

Vol. 26, no. 4

649--656

l-Deprenyl and its major metabolites were subjected to reversed-phase high-performance liquid chromatography (HPLC). The separation was monitored using both ultraviolet (UV) and electrochemical detectors. Ultraviolet absorbance detected l-deprenyl, l-nordeprenyl, l-methamphetamine, and l-amphetamine. Amperometric detection was specific and sensitive to the parent compound (l-deprenyl, a tertiary amine) only. Peaks of the major L-deprenyl metabolites did not give any comparable signal using amperometric monitoring.

Comparative analysis of six different brands of date fruits

Kursinszki L., Kalász H., Szőke É, Adeghate E, Hassan M. Y, Adem A

Acta Chromatographica

2011

Vol. 23, no. 4

603--610

Flavonoid composition of six different brands of Phoenix dactylifera L. date fruit (Lulu, Khalas, Khenaiz, Al-Medina, Razaiz, and Fardh) was identified, determined, and compared. The separation was done on a Zorbax Eclipse XDB-C18 (150 × 4.6 mm ID) reversed-phase column using gradient elution of 1% aqueous formic acid (A) and acetonitrile (B). Gradient conditions were 00.00 min, 13% B; 20.00 min, 34% B; 25.00 min, 64% B; 29 min, 64% B; 35 min, 13% B; 39 min, 13% B. Monitoring was done using ultraviolet absorbance and MS/MS.