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1

Corrosion behaviour of polished and sandblasted titanium alloys in PBS solution

Burnat B., Walkowiak-Przybyło M., Błaszczyk T., Klimek L.

Acta of Bioengineering and Biomechanics

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2013

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Vol. 15, no. 1

87--94

EN

In this work, we performed comparative studies of the effect of surface preparation of Ti6Al4V and Ti6Al7Nb biomedical alloys and the influence of endothelial cells on their corrosion behaviour in PBS (Phosphate Buffered Saline). Two different methods of surface modification were applied – polishing and sandblasting. The polished Ti6Al7Nb alloy was found to have the best resistance against general corrosion in PBS. It was characterized by the lowest corrosion rate, the widest passive range and the lowest reactivity. Both alloys prepared by sandblasting exhibited worse corrosion properties in comparison to the polished ones. This can be associated with a greater development of their surface and the presence of Al2O3 grains which caused an increase of corrosion potential but might also influence the weakening of the passive layer. Results of potentiodynamic anodic polarization indicated that more resistant to pitting corrosion was Ti6Al7Nb alloy regardless of the method of surface preparation. In those cases, anodic polarization caused only an increase of passive layer, while in the case of sandblasted Ti6Al4V alloy it caused a pitting corrosion. The results obtained allowed us to conclude that the niobium-titanium alloys had higher corrosion resistance than titanium alloys with vanadium. Moreover, it was stated that endothelial cells improved the corrosion resistance of all the titanium alloys examined.

2

Analiza zmian w ekspresji genów cyklu komórkowego w komórkach osteoblastów hodowanych na powierzchniach stopów tytanu

Walkowiak-Przybyło M., Komorowski P., Jakubowski W., Klimek L., Walkowiak B.

Engineering of Biomaterials

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2012

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Vol. 15, no. 116-117 spec. iss.

46--48

3

Proliferacja osteoblastów Saos-2 oraz komórek endotelialnych EA.hy 926 na polerowanych i piaskowanych powierzchniach stopów tytanu Ti6Al4V i Ti6Al7Nb

Walkowiak-Przybyło M., Walczyńska M., Komorowski P., Klimek L., Walkowiak B.

Engineering of Biomaterials

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2011

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Vol. 14, no. 109-111 spec. iss.

39--41

4

Ocena trombogenności powierzchni polerowanego stopu tytanu Ti6Al7Nb modyfikowanego cienką warstwą fosforanu wapnia

Walkowiak-Przybyło M., Okrój W., Walczyńska M., Skwierczyński P., Walkowiak B.

Engineering of Biomaterials

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2011

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Vol. 14, no. 109-111 spec. iss.

37--38

5

Biofilm formation under anaerobic conditions on biomaterial’s surface

Jakubowski W., Biela T., Kamińska M., Walkowiak-Przybyło M., Walkowiak B.

Engineering of Biomaterials

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2009

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Vol. 12, no. 89-91

253

EN

Investigations of the biofilm formation processes on biomaterials surfaces are focused on typical physiologic conditions - aerobic condition. In experimental works onto model strains, and also in the clinical reports there is a lack of the information related to the mechanisms engaged in biofilm grown under conditions of limited access to oxygen or in entirely anaerobic environment. Presented results show the colonization of medical steel 316L surface and creation of biofilm structures by E. coli bacteria’s, under oxygen deficit conditions or its total lack. In studied arrangement the change of the dynamics of bacterium growth was observed, which led to considerable slowdown of the biofilm development. Bacteria were observed with use of the combination of fluorescent dyes - bis-benzimide and propidium iodide – which makes it possible the distinction of live/death bacteria. In oxygen deficiency and in anaerobic conditions multiplication of death bacteria level in comparison to standard conditions was observed (suitably 34% and 41% dead cells, oxygenic conditions - 1%). The limitation of oxygen utilization possibility by the bacteria, which colonized investigated surfaces also resulted in decreased sizes reached by the cells.

6

Comparison of microscopic methods for evaluating platelet adhesion to biomaterial surfaces

Okrój W., Walkowiak-Przybyło M., Rośniak-Bąk K., Klimek L., Walkowiak B.

Acta of Bioengineering and Biomechanics

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2009

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Vol. 11, nr 2

45-49

EN

Microscopic methods usable for sample surface imaging and subsequent qualitative and quantitative evaluation of platelet adhesion to the surface of the biomaterial studied were compared. It was shown, making use of the samples of medical steel (AISI 316L), that such tools as surface imaging with scanning electron microscopy (SEM), glutaraldehyde induced fluorescence technique (GIFT) and metallurgical microscopy (MM) are equivalent in evaluating surface platelet adhesion. The importance of biological variability of blood samples for a proper result assessment and the necessity of using internal standards were also considered.