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1 Materials Science Poland

1 2023

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Antimicrobial activity, viability, and physicochemicalproperties of an MTA-type cement with differentconcentrations of bismuth trioxide

Martínez-Hernández Miryam, Manzo-García Daniel, Ramírez-Ortega Juana Paulina, Arreguín-Cano Juan Antonio, Chávez-Cortéz Elda Georgina, Arenas-Alatorre Jesús Ángel, Rodríguez-Chávez Jacqueline Adelina, Flores-Ledesma Abigailt

Materials Science Poland

2023

Vol. 41, No. 2

339--353

In medicine, bismuth is used as an antimicrobial agent. In dentistry, it is used primarily to increase radiopacity in someendodontic materials. The objective is to evaluate the antimicrobial activity, cell viability, pH, solubility, film thickness, andsetting time of a mineral trioxide aggregated (MTA)-types of cement with different concentrations of bismuth trioxide. Threeexperimental MTA-types of cement with a bismuth trioxide (Bi2O3) concentration of 15 wt%, 20 wt%, and 25 wt% were used.The antimicrobial activity test was conducted on Streptococcus mutans and Porphyromonas gingivalis strains. Cell viability wasmeasured by the quantitative colorimetric assay using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide)assay on a mouse fibroblast cell line (L929). Solubility, film thickness, and setting time were performed according to ISO 6876.The lowest Bi2O3concentrations showed the best antimicrobial activity and cell viability. pH, solubility, setting time, and filmthickness did not show statistically significant differences between the different Bi2O3concentrations tested.