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1 Physicochemical Problems of Mineral Processing

1 2015

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Hydroxyapatite as a support in protease immobilization process

Zdarta J., Budzinska K., Kolodziejczak-Radzimska A., Klapiszewski Ł., Siwinska-Stefanska K., Bartczak P., Piasecki A., Maciejewski H., Jesionowski T.

Physicochemical Problems of Mineral Processing

2015

Vol. 51, iss. 2

633--646

Hydroxyapatite is used as a matrix for immobilization of protease from Aspergillus oryzae by a process of adsorption. The matrix obtained has the surface area of 26 m2/g and particles in the shape of flakes of diameters no greater than 650 nm. The efficiency of the proposed method was confirmed by the Fourier transform infrared spectroscopy, elemental analysis and by analysis of parameters of the pore structure of matrix and products after immobilization. On the basis of the Bradford method it was found that the greatest amount of enzyme (132 mg/g) was immobilized from a solution of initial enzyme concentration of 7 mg/cm3 after 24 h of the process.