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Preparation and anatomical distribution study of 67Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)

Heidarieh M., Daryalal F., Mirvaghefi A., Rajabifar S., Diallo A., Sadeghi M., Zeiai F., Moodi S., Maadi E., Sheikhzadeh N., Heidarieh H., Hedyati M.

Nukleonika

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2014

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Vol. 59, No. 4

153--159

EN

Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic acid) in fi sh. Therefore, feasibility of developing alginic acid nanoparticles to detect target organ in rainbow trout is interesting. To make nanoparticles, Ergosan extract (alginic acid) was irradiated at 30 kGy in a cobalt-60 irradiator and characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results from TEM images showed that particle sizes of irradiated alginic acid ranged from 30 to 70 nm. The FTIR results indicated that gamma irradiation had no signifi cant infl uence on the basic structure of alginic acid. Later, alginic acid nanoparticles were successively labelled with 67Ga-gallium chloride. The biodistribution of irradiated Ergosan in normal rainbow trout showed highest uptake in intestine and kidney and then in liver and kidney at 4- and 24-h post injection, respectively. Single-photon emission computed tomography (SPECT) images also demonstrated target specifi c binding of the tracer at 4- and 24-h post injection. In conclusion, the feed supplemented with alginic acid nanoparticles enhanced SPECT images of gastrointestinal morphology and immunity system in normal rainbow trout.

2

Preparation and biodistribution of 99mTc-IgG-HYNIC in normal rats

Rajabifar S., Akhlaghi M., Jalilian A. R., Bolourinovin F., Maashkar B., Talebimehrdar M., Ghafouri M.

Nukleonika

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2009

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Vol. 54, No. 4

279-284

EN

Human gamma globulin can be labeled by a direct or indirect method of radiotracer incorporated in a protein molecule. In this indirect method hydrazinonicotinic acid (HYNIC) is used which saves the structure and biological activity of the protein. Our goal was the efficient labeling of the human gamma globulin and evaluation of its biodistribution in different organs which can be used on experimentally induced infection causing inflammation. Immune globulin is mixed with s-hynic and IgG-hynic is developed using sidle A-lyzer and stored at –20°C which can be used at least for six months and then Sn-tricine kit is prepared which is used for 99mTc labeling. Efficiency of 99mTc-IgG-hynic labeling at pH 6.4 was very much dependent on ligand (hynic) and coligand (tricine) presence in the reaction mixture. Radiochemical purity was more than 90% in the kits prepared. Serum stability study showed no decomposition of 99mTc from the complex. The biodistribution studies showed the highest percentage ID/organ in the blood, liver and kidney, respectively. A human gamma globulin was successfully labeled through hynic to 99mTc by an indirect method with high radiochemical purity.

3

Cyclotron production of 68Ga via proton-induced reaction on 68Zn target

Sadeghi M., Kakavand T., Rajabifar S., Mokhtari L., Rahimi-Nezhad A.

Nukleonika

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2009

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Vol. 54, No. 1

25-28

EN

68Ga is an important positron-emitting radionuclide for positron emission tomography. In this work 68Ga was produced via the 68Zn(p,n)68Ga nuclear reaction. 68Zn electrodeposition on a copper substrate was carried out by alkaline cyanide baths. 68Zn target was irradiated with a 15 MeV proton beam and a 150 mi A current. The production yield achieved was 136 mCi/ mi Aźh (5.032 GBq/mi Aźh). 68Ga was separated from zinc and copper by a combination of cation exchange chromatography and liquid-liquid extraction methods.

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Preparation and biodistribution of [67Ga]-insulin for SPECT purposes

Jalilian A. R., Garousi J., Gholami E., Akhlaghi M., Bolourinovin, Rajabifar S.

Nukleonika

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2007

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Vol. 52, nr 4

145-151

EN

Human recombinant insulin was successively labeled with [67Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (ccDTPA). The best results of the conjugation were obtained by the addition of 0.5 ml of an insulin pharmaceutical solution (5 mg/ml, in phosphate buffer, pH = 8) to a glass tube precoated with DTPA-dianhydride (0.01 mg) at 25°C with continuous mild stirring for 30 min. Radiothin-layer chromatography (RTLC), instant thin-layer chromatography (ITLC) and high-performance liquid chromatography (HPLC) showed overall radiochemical purity higher than 96% in optimized conditions (specific activity = 300 500 MBq/mg, labeling efficiency 77%). Preliminary in vivo studies with normal rats were performed to determine the biodistribution of the radiotracer up to 110 h. They showed a high liver uptake of the tracer which is consistent with other reported radiolabeled insulins.

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Development of 111In-DTPA-human polyclonal antibody complex for long-term inflammation/infection detection

Jalilian A., Rowshanfarzad P., Shafaii K., Kamali-Dehghan M., Moafian J., Akhlaghi M., Babaii M., Rajabifar S., Mirzaii M.

Nukleonika

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2005

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Vol. 50, nr 3

91-96

EN

Human polyclonal antibody (HIgG) was successively labeled with 111In-indium chloride after residulation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of solid DTPA-dianhydride (0.1 0.3 mg) to 100 mi l of the HIgG solution (0.2 0.4 mg/ml) at pH = 6 in phosphate buffer media at 25°C with continuous stirring for 30 min. Radio-thin-layer chromatography showed an overall radiochemical yield of 96 99% at optimized conditions (specific activity = 300 500 MBq/mg, radiochemical purity >98%). The final isotonic 111In-DTPA-HIgG complex was checked by radio-TLC to ensure the formation of only one species followed by filtration through a 0.22 mi filter. Preliminary long-term in vivo studies in turpentine-oil induced inflammation in rat model was performed to determine late complex distribution of the radioimmunoconjugate. The target/skin and target/ blood ratios were 27 and 51 after 24 h, and 23 and 51 after 110 h, showing a high selectivity of the radiopharmaceutical for inflammatory lesions.