Wyniki wyszukiwania - BazTech

Ograniczanie wyników

Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Formation and preclinical evaluation of a new alloplastic injectable bone substitute material

Bojar W., Kucharska M., Bubak G., Ciach T., Koperski Ł., Jastrzębski Z., Gruber B.M., Krzysztoń-Russjan J., Marczewska J., Anuszewska E., Drozd E., Brynk T.

Acta of Bioengineering and Biomechanics

2012

Vol. 14, no. 1

39-44

Alloplastic bone substitute materials are raising some more interest as an alternative for autologic transplants and xenogenic materials especially in oral surgery over the last few years. These non-immunogenic and completely resorbable biomaterials are the basis for complete and predictable guided bone regeneration. In the majority of cases, such a material is chosen because of its convenient application by surgeons. The main objective of our project was to design and fabricate an osteoconductive, injectable and readily tolerable by human tissues biomaterial for guided bone regeneration. For this purpose, a self-setting composite consisting of chitosan/tricalcium phosphate microparticles and sodium alginate was made. The material obtained was characterized by microsphere and agglomerate morphology and microstructure. Its features relating to setting time and mechanical properties were precisely investigated. Our material was also evaluated according to PN-EN ISO 10993 Biological evaluation of medical devices, i.e., the in vitro tests for genotoxicity and cytotoxicity were conduced. Then, the following examinations were performed: subchronic systemic toxicity, skin sensitization, irritation and delayed-type hypersensitivity and local effects after implantation. The material tested showed a high degree of cytocompatibility, fulfilled the requirements of International Standards and seemed to be a "user friendly" material for oral surgeons.

Badania wpływu Selolu i seleninu sodu na komórki HeLa in vitro

Dudkiewicz-Wilczyńska J., Książek I., Nowak K., Suchocki P., Flis S., Kiljan M., Anuszewska E.

Chemik

2011

Vol. 65, nr 2

105-114

Zaprezentowano wyniki z badań nad antyproliferacyjnym działaniem Selolu 5% na komórki nowotworowe HeLa, w odniesieniu do seleninu sodu. Na podstawie uzyskanych wyników w teście MTT, zawartości białka całkowitego, indukcji apoptozy i penetracji selenu do komórek, stwierdzono zależne od czasu inkubacji cytotoksyczne działanie Selolu, (IC50 ~ 80 ?MSe, 48 h), w porównaniu z silnym działaniem cytotoksycznym seleninu sodu (IC50 ~ 5,9 ?M Se, 24 h). Powolna penetracja selenu z Selolu do komórki nowotworowej, wpływająca na przedłużony czas jego działania, umożliwia potencjalne wykorzystanie Selolu w terapii przeciwnowotworowej.

The paper presents results of studies on the antiproliferative effect of Selol 5% on HeLa tumour cells, as compared to sodium selenite. Based on the results of the MTT test, determination of total protein, apoptosis induction and penetration of selenium into the cells, an incubation time-dependent cytotoxic activity of Selol was established (IC50 ∼80 μM Se, 48 h), and compared to the strong cytotoxic activity of sodium selenite (IC50 ∼ 5,9 μM Se, 24 h). Slow penetration of selenium from Selol into the tumour cell, responsible for the prolonged time of its activity, enables potential application of Selol in antitumour therapy.

Capillary electrophoresis for the determination of N-Acetyltyrosine and N-acetylcysteine in products for parenteral nutrition: Method development and comparison of two CE systems

Jaworska M., Szulińska Z, Wilk M., Anuszewska E.

Acta Chromatographica

2011

Vol. 23, no. 4

595--602

Because of the problems with stability and solubility, some of the amino acids (AAs) used in parenteral nutrition products are often replaced by their acetylated forms. These include N-acetyltyrosine (NAT) and N-acetylcysteine (NAC). This leads to a need to develop new analytical methods for rapid and easy determination of these substances in the presence of common AAs. Capillary electrophoresis (CE) is one of the techniques that have been successfully applied to the assay of multi-component samples containing AAs and their derivatives. This paper discusses a new CE method for the simultaneous determination of two acetylated AAs in solutions for parenteral nutrition. A background electrolyte (BGE) was developed based on borate buffer with alkaline pH. The method is selective and enables the separation and assay of analytes without special sample pretreatment. Validation parameters confirmed sufficient precision and accuracy of the method. Its applicability was verified by testing several medicinal products from various manufacturers. Moreover, the flexibility of the method was checked using two brands of CE equipment. Appropriate adjustment of instrumental parameters turned out to be essential for method transferability. The method could be used in routine testing of parenteral nutrition medicinal products containing AAs.