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Selective RP-HPLC method for determination of quetiapine in presence of coadministered drugs: Application for long-term stability study of quetiapine in whole blood

Youssef R. M., Abdine H., Barary M. A., Wagih M. M.

Acta Chromatographica

2016

Vol. 28, no. 3

263--279

Incidence of suicidal attempts presents an explanation for the high prevalence of quetiapine (QTI) in forensic cases. Thus, the interpretation of its concentrations in biological specimens is needed, but in forensic toxicology, potential postmortem changes such as instability of the target drugs should be taken in consideration. High-performance liquid chromatography (HPLC) method has been developed for determination of QTI. This method was based on reversed phase (RP)-HPLC separation of QTI on a C-18 column (150 mm × 4.6 mm, 5 μm) with elution system of acetonitrile—methanol—0.025 M phosphate buffer (pH 2.5), containing 1 mL TEA in each 250 mL, in a ratio of 40:30:30%, v/v, at the flow rate of 1.2 mL min−1 using mirtazapine as internal standard (IS). The proposed method was applied to the determination of QTI in plasma in presence of coadministered drugs. The application of the proposed method was extended for long-term stability study of two different concentration levels of QTI in the whole blood.

Development of gradient HPLC-DAD method for assay of ternary mixture containing amebicide and analgesic drugs

Youssef R. M.

Acta Chromatographica

2014

Vol. 26, no. 1

67--80

Validated gradient high-performance liquid chromatography-diode array detection (HPLC-DAD) method has been developed for simultaneous determination of diloxanide furoate (DIL), mebeverine hydrochloride (MBV), and metronidazole (MET) in their pharmaceutical preparation. This method was based on a reversed-phase HPLC separation of the cited drugs on a C-18 column (150 mm × 4.6 mm, 5 μm) with a gradient elution system of 0.05 M phosphate buffer with 1% (v/v) of triethylamine adjusted to pH 3.0-methanol as the mobile phase at the flow rate of 1.0 mL min -1. The separation was carried out at ambient temperature. Quantitation was achieved with DAD detection at 260 nm for DIL and MEB, and at 320 for MET based on peak area with linear calibration curves at concentration ranges 4–30, 1–20, and 1–30 μg mL -1 for DIL, MEB, and MET, respectively. The proposed chromatographic method was successfully applied to the determination of the investigated drugs in pharmaceutical preparation. It was validated in compliance with International Conference on Harmonization (ICH) guidelines, in terms of linearity, accuracy, precision, robustness, limits of detection, and quantitation and other aspects of analytical validation.