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1 2011

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Study of retention of 31 polyoxygenated steroids by normal- and reversed-phase thin-layer chromatography

Tosti T, Natić M, Smoliński A., Milić D, Milojković-Opsenica D, Tešić Ž

Acta Chromatographica

2011

Vol. 23, no. 3

429--445

The chromatographic behavior of 31 steroidal compounds on both silica gel and RP-18 silica thin layers using nonaqueous and aqueous-organic mobile phases was investigated. It was found that an application of more polar solvent systems such as acetonitrile-dichloromethane in comparison to acetone-n-hexane resulted in stronger elution of the investigated substances in all investigated cases. Under normal-phase conditions better selectivity was achieved in acetonitrile-dichloromethane and the best in binary mixture with component ratio 1:9 (υ/υ). The selectivity of the separation under reversed-phase conditions was increased with the increased fraction of water in mobile phases used. A conclusion on the influence of substance moiety on the retention behavior of the investigated substances under reversed-phase conditions could not be drawn due to complex retention mechanism. In order to find similarities and dissimilarities between normal- and reversed-phase systems used, retention parameters were investigated by principal component analysis and cluster analysis.