A simple, specific, and precise RP-HPLC method with photodiode-array detection has been developed for simultaneous analysis of the quinazoline alkaloids vasicine and vasicinone, pharmacologically important constituents of Adhatoda vasica . The compounds were separated by use of 0.1% trifluoroacetic acid in water:acetonitrile 90:10 as isocratic mobile phase at a flow rate of 1.0 mL min -1 Under these conditions, plots of integrated peak area against concentration were linear over the ranges 0.05–10.0 μg mL -1 and 0.5–100.0 μg mL -1 ( r 2 = 0.999 and 0.998) for vasicine and vasicinone, respectively. Mean recovery was 99.06% for vasicine and 98.69% for vasicinone. The method is therefore suitable for routine analysis of vasicine and vasicinone in Adhatoda vasica plants of different varieties and in herbal medicinal products prepared from the plants.
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