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2 Acta Chromatographica

2 2010

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Simultaneous RP-HPLC analysis of atenolol, hydrochlorothiazide, and losartan potassium in a tablet formulation

Thomas A. B, Chavan U. B, Nanda R. K, Kothapalli L. P, Jagdale S. N, Dighe S. B, Deshpande A. D

Acta Chromatographica

2010

Vol. 22, no. 2

219--226

Simultaneous analysis of atenolol (Atn), hydrochlorothiazide (Hctz) and losartan potassium (Los) in solid dosage forms has been achieved by reversed-phase high-performance liquid chromatography on a C 18 column with a 0.035 M potassium dihydrogen orthophosphate-acetonitrile gradient as mobile phase and UV detection at 225 nm. The retention times for Atn, Hctz, and Los were 2.91, 4.75, and 7.52 min, respectively, with mean recoveries of 99.67, 99.89, and 100.69%. The method was validated in accordance with ICH guidelines. Because of its simplicity and high precision and accuracy, the method can be used for analysis of atenolol, hydrochlorothiazide and losartan potassium in pharmaceutical preparations.

Development and validation of a method for densitometric analysis of lupeol from Mimosoups elengi

Ganu G. P, Jadhav S. S, Deshpande A. D

Acta Chromatographica

2010

Vol. 22, no. 3

491--497

In this paper we describe a simple, precise, and accurate HPTLC method for quantification of lupeol in the bark of Mimosoups elengi (ME). The bark was extracted with hot methanol in a Soxhlet apparatus. Chromatographic separation of the extract was performed on aluminum foil plates coated with silica gel 60 F 254 as the stationary phase. The mobile phase was toluene-ethyl acetate-formic acid 12:2:1 ( υ/υ ). Densitometric evaluation of the separated zones was performed at 220 nm. The lupeol was satisfactorily resolved at R F 0.64 ± 0.02. The accuracy and reliability of the method were assessed by evaluation of linearity (1000–4000 ng per band), precision (method and instrumental precision, as RSD, 1.06 and 1.03%, respectively), accuracy (97.28 ± 0.89), and specificity in accordance with ICH guidelines.