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1 Acta Chromatographica

1 1998

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Paper-electrophoretic studies of (-amino acids in citrate-phosphate buffer and sodium dodecyl sulphate mixed systems

Nabi S. A., Usmani S., Rahman N.

Acta Chromatographica

1998

No. 8

175-189

The electrophoretic behaviour of 21 amino acids has been studied on Whatman No.1 paper strips in citrate-phosphate buffer systems of different pH. The role of sodium dodecyl sulphate (SDS) with citrate-phosphate buffer on the mobility of amino acids has been critically observed. It has been found that the presence of SDS in the citrate-phosphate buffer significantly enhances the separation possibilities. On the basis of the different migration of the amino acids, several important qualitative and quantitative separations have been achieved with these electrolyte systems. The practical utility of the method has been demon-strated by quantitative separation and determination of the amino acid constituents of the standard drugs Santevini (plus) and Astymin-3.