Coronavirus (COVID-19) was detected at the end of 2019 and has since caused a worldwide pandemic. This virus is transferred airborne. In this study, an investigation was carried out of the ventilation strategies inside the isolation room based on exhaust air locations. To reduce the infection disease (COVID-19), due to the spreading of exhaled contaminants by humans in interior environments, five models for ventilation systems differing in the position of the outlet and inlet were used. This study aims to increase knowledge regarding the exhaled contaminant distribution under different environ-mental conditions (opening exhaust and negative pressure). The results showed a good agreement be-tween the computational results and the experimental data. Tracer gas CO2 was used to evaluate the air quality experimentally and computationally. The results showed that stable conditions are obtained inside the room at a negative pressure value above –1.5 Pa. The residence time of the infected airborne decreases when the pressure difference increases. The study revealed that the model with an air outlet opening installed behind the patient enabled avoiding the spread of infection in the room.
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