Palm (Phoenix dactylifera L.) is a specie cultivated in Mauritania. The present study is focused on the potential of the aqueous extract of the Pergularia tomentosa L., as biological control agent against Fusarium brachygibbosum, the main agent of the date palm stipe rot disease in Mauritania. Fungal pathogen was isolated from symptomatic date palm stipe rot disease in Mauritania. The morphological characterization and the molecular identification by sequencing ITS1, ITS2 5.8 RNAr region showed homology of 98% with Fusarium brachygibbosum strain UOA/HCPF 16982 s during in vitro tests on leaves performed to verify its phytopathogenicity. The inhibitory effect of aqueous extract of Pergularia tomentosa L. on the phytopathogenic isolate (Fusarium brachygibbosum) of date palm, on mycelial growth and spore germination was observed. In fact, in vitro on PDA, mycelial growth ranged from 39.23 to 67.7%, depending on the concentration of the aqueous extract of P. tomentosa. The aqueous extract showed a reduction of spore germination varying from 5.3 to 23.8%. The minimum inhibitory concentrations varied from 1 to 100 mg/ml. The various extracts give high antioxidant activities exhibited by DPPH assay. Multivariant analysis by PCA plot and the heatmap were done, to evaluate the correlation between the tested parameters. These results suggest the use of Pergularia tomentosa L., extract as a biological agent to control and reduce damage caused by Fusarium brachygibbosum.
Awleigatt National Park (ANP) is a zoological site ensuring both the conservation and restoration of biodiversity on a national scale. In this perspective, the ANP has set as one of its ambitious objectives the autonomy in fodder needs. The objective of this work was to study the adaptability of three forage species (Medicago sativa, Acacia senegal and Dolichos lablab) to the edaphic conditions of the NAP. The adopted approach was to characterize three types of representative ANP soil substrates in order to develop an interpolation map of EC conductivity and pH data. Subsequently, the germination behavior of the three species was studied in the laboratory on three substrates taken from the study area (Dune, Dune flank and Interdune) in the absence and presence of NaCl (0, 50 and 100 Mm). The obtained results show that the soils are basic and the electrical conductivity is higher in the inter-dune substrate. The mapping of the study area corroborates the experimentally obtained data. Germination behavior shows that germination is best on dune and dune flank substrates. The addition of high concentrations of NaCl (100mM) shows that Dolichos lablab is the most resistant to salinity. Medicago sativa and Acacia senegal show low germination capacity both in the absence and in the presence of NaCl, most likely reflecting a low embryonic longevity of the seeds used. The parameters deduced from the germination kinetics curve show that the adverse effect of salinity in the three species is observed at all three germination phases (TL, GSS and CG). As a result, the effect of salinity on germination of all three species is both osmotic and toxic.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.