Phenol is an internal browning (IB) enzymatic reaction substrate and endogenous abscisic acid (ABA) used to suppress IB incidence in the Comte de Paris cultivar (Queen type). There is no information on the correlation between pineapple IB to endogenous total phenol content (TPC), ABA, and gibberellic acid 3 (GA3) after postharvest applications of decrowning. Therefore, this research aimed to analyze the relationship of IB incidence to total TPC, ABA, and GA3 after postharvest treatments of decrowning and coating in GP3 and MD2 pineapple clones. The structure was based on a completely randomized design with 3 factors, namely clone (GP3 and MD2), decrowning (crown and crownless), and coating [50 mg L-1 ABA, 1% chitosan, ABA+Chitosan mixture, and control (H2O)]. The results showed that the MD2 had a lower IB incidence and higher TPC than the GP3 stored at 7°C for 37 days. The increased TPC was positively correlated with IB incidence. TPC was also negatively correlated with ABA but positively with endogenous GA3 2 weeks earlier. Coating with 50 mg L-1 ABA and 1% chitosan on MD2 decreased IB incidence. Pineapple crown pruning decreased ABA and increased TPC, GA3, and IB incidence.
The shelf life of pineapple is significantly influenced by storage temperature and can be prolonged by maintaining an optimal temperature range of 5-12°C. However, there is still the problem of internal browning (IB) in the long-term storing of fresh harvest at cold temperatures. Postharvest application of 380 μM ABA (Abscisic Acid) to the crown, which is a source of ABA endogenous was found to suppress IB, while the concentration of 95 μM was not effective. Therefore, this research aimed to determine the response of GP3 and MD2 clones to postharvest treatment with the application of 50 mg/L ABA, chitosan and decrowning on the IB severity and other fruit qualities. The experimental design used a Completely Randomized Design with 3 factors of clone (GP3 and MD2), decrowning (crown and crownless), and fruit coating [chitosan 1%, ABA 50 mg/L, ABA + chitosan mix, and control (H2O)]. The fruits were kept at 7°C and observed at 0, 3, 6, 9, 16, 23, 30, and 37 days. The results showed that MD2 was significantly lower IB than GP3 and IB severity negatively correlated with ascorbic acid (AsA) content. MD2 had lower fruit weight loss (FWL) and skin dehydration (SD), higher AsA, soluble solid content (SSC), and SSC/titratable acidity (STA) ratios compared to GP3. The crown + ABA treatment decreased the IB severity of GP3, with a level of 0.75% after 37 days which was lower than crown + H2O by 9.17% and crownless + H2O by 8.42%. ABA treatment also showed higher SD and FWL, while AsA, SSC, TA, and STA were not different from the control.
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