Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Acta Chromatographica

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies

Li J. R., Li D. X., Li L., Deng W. L., Ding L. S., Xu H. X., Zhou Y.

Acta Chromatographica

2014

Vol. 26, no. 4

711--725

A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295→249, 191 for tanshinone IIA and m/z 297→279, 251 for IS in positive mode, m/z 717→519, 321 for salvianolic acid B and m/z 295→267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL-1 ng/mL for tanshinone IIA and 100 pg/mL-1 for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.

Simultaneous HPLC-DAD analysis of four methylated flavonols in Amomum koenigii

Qiao C. F., Han Q. B., Song J. Z., Yiu H., Xu H. X.

Acta Chromatographica

2007

No. 19

217-226

Four bioactive methylated flavonols isolated from Amomum koenigii J.F. Gmelin, 3,7-dihydroxy-5,3',4'-trimethoxyflavone (1), 5-hydroxy-3,7,4'-trimethoxyflavone (2), 3,7-dihydroxy-5,4'-dimethoxyflavone (3), and 5-hydroxy-3,7,3',4'-tetramethoxyflavone (retusin, 4), have been analyzed by HPLC–DAD on a 4.6 mm × 150 mm, 5-µm particle, C8 column with an acetonitrile–aqueous acetic acid gradient as mobile phase. UV detection was at 360 nm. The method was validated in detail. The results indicated that the total amounts of these four constituents in the seeds were 0.776–0.829%, higher than in the pericarp. Retusin was the major constituent with a maximum content of approximately 0.50% in the seeds. This method is useful for quality control of the crude drug.