Furanocoumarins from Peucedanum tauricum Bieb. and their variability in the aerial parts of the plant during development

• Autorzy: Bartnik M. , Głowniak K.
• Konferencja: 5th International Symposium on Chromatography of Natural Products ISCNP (19-22.06.2006 ; Lublin, Poland)
• Języki publikacji: EN

Abstrakty

EN

A rapid and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been used for determination of furanocoumarins in methanolic extracts of Peucedanum tauricum Bieb. HPLC separation was performed on a C18 analytical column (250 mm × 4 mm i.d., 5-μm particles) with a methanol–water gradient as mobile phase. A second chromatographic system with an acetonitrile–water gradient was also developed independently. Diode-array detection was performed between 220 and 400 nm and in quantitative analysis compounds were detected at 320 nm. Calibration plots were linear for 0.01–0.2 mg mL-1 bergapten and peucedanin in methanol (injection volume 10 μL). Before HPLC, samples were purified by solid-phase extraction (SPE). Oxypeucedanin hydrate (oPh), bergapten (5-MOP), oxypeucedanin (oP), peucedanin (P), 8-methoxypeucedanin (mP), and isoimperatorin (isoIMP) were detected and identified in extracts of P. tauricum. Total amounts of furanocoumarins determined by SPE–HPLC were 8.31 mg g-1 dry wt (0.83%) in flowers, 20.49 mg g-1 dry wt (2.05%) in immature fruits, and 21.30 mg g-1 dry wt (2.13%) in mature fruits. The predominant coumarin compound in the reproductive organs was peucedanin – 5.04 mg g-1 dry wt (0.50%) in flowers, 13.58 mg g-1 dry wt (1.36%) in immature fruits, and 13.51 mg g-1 dry wt (1.35%) in completely mature fruits. The predominant furanocoumarin in the leaves, however, was 8-methoxypeucedanin – from 0.55 mg g-1 to 1.02 mg g-1 dry wt (0.06–0.10%). The furanocoumarin content of the plant extracts correlated with period of development and the part of the plant (generative or vegetative). Some of the detected compounds (e.g. peucedanin and 8- methoxypeucedanin, which are both linear furanocoumarins of a rare structural type with restricted occurrence in P. officinale and the closely related species P. coriaceum and P. longifolium) could be a chemotaxonomically characteristic of the species investigated. As we have reported elsewhere, peucedanin is effective at induction of apoptosis and inhibition of heatshock protein expression in HeLa cells. The fruits of P. tauricum are a good source of peucedanin for further biological and pharmacological tests.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2007
• Tom: No. 18
• Strony: 5--14
• Opis fizyczny: Bibliogr. 18 poz., rys., tab.

Twórcy

autor

Bartnik M.

autor

Głowniak K.

• Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University, 1, Chodźki Str., 20-093 Lublin, Poland

Bibliografia

• [1] R.D.H. Murray, J. Mendez, and S.A. Brown, The Natural Coumarins, Wiley, Chichester, 1982
• [2] R.D.H. Murray, Naturally Occurring Plant Coumarins, Springer, Vienna, 1991
• [3] T.G. Tutin, V.H. Heywood, N.A. Burges, D.M. Moore, D.H. Valentine, S.M. Walters, and D.A. Webb, Flora Europaea. Vol 2 (Rosaceae to Umbelliferae), University Press, Cambridge, 1968
• [4] A.A. Grossgiejm, Flora Kawkaza. Vol. VII. Umbelliferae – Scrophulariaceae, Izdatielstwo Nauka, Leningrad, 1967
• [5] B.K. Szyszkin, Flora SSSR, Vol. XVII, Izdatielstwo Akademii Nauk SSSR, Moskwa, Leningrad, 1951
• [6] D.I. Baranauskeite and G.K. Nikonov, Apotech. Delo, 14, 25 (1965)
• [7] K. Głowniak, M. Bartnik, T. Mroczek, A. Zabża, and A. Wierzejska, J. Planar Chromatogr., 15, 94 (2002)
• [8] M. Bartnik, Doctoral dissertation, Medical University, Lublin, 2004, p. 242
• [9] H. Tesso, W.A. König, K.-H. Kubeczka, M. Bartnik, and K. Głowniak, Phytochemistry, 66, 707 (2005)
• [10] M. Bartnik, K. Głowniak, and R. Dul, J. Planar Chromatogr., 16, 206 (2003)
• [11] M. Bartnik, K. Głowniak, and M. Mardarowicz, Acta Pol. Pharm.–Drug Res., 56, 457 (2002)
• [12] M. Bartnik, K. Głowniak, and A. Gromek, J. Planar Chromatogr., 20, 127 (2007)
• [13] Polish Pharmacopoeia VI, PTFarm, Warsaw, 2002
• [14] G. Zgórka and K. Głowniak, Phytochem. Anal., 10, 268 (1999)
• [15] F. Hadaček, Stapfia, 18, 1 (1989)
• [16] F. Hadaček, C. Müller, A. Werner, H. Greger, and P. Proksch, J. Chem. Ecol., 20, 2035 (1994)
• [17] M. Bartnik, K. Głowniak, J. Jakubowicz-Gil, B. Pawlikowska-Pawlęga, and A. Gawron, Phytotherapy – The Role of an Ancient Tradition in Modern Times, 1–5 Nov. 2005, Madeira, Portugal, P62, p. 132
• [18] M. Bartnik, K. Głowniak, J. Jakubowicz-Gil, B. Pawlikowska-Pawlęga, and A. Gawron, Herba Polonica, 52, 58 (2006)