PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water

• Autorzy: Ziembińska-Buczyńska A. , Cema G. , Szpindor M. , Meresta A. , Płonka L.
• Języki publikacji: EN

Abstrakty

EN

Reject water, produced during stabilization and dewatering of activated sludge, contains a high load of biogenic compounds which are returned to bioreactors, so there is a necessity for its treatment within biological systems. The efficacy of such performance depends mainly on the activated sludge composition responsible for the biochemical processes. For biocenosis diversity and changeability, PCR-DGGE is the most commonly used tool. In this article we monitored the activated sludge bacterial communities of two sequencing batch reactors (SBRs) dealing with reject water but varying in their operational parameters. A physico-chemical analysis of the SBRs performance was also done. We used two different PCR primers sets to present a total overview of the changeability in complex microbial biocenosis during wastewater treatment. For biodiversity monitoring 16S rRNA gene fragments amplified with 338f-GC/518r primers appeared to be more suitable than the fragments amplified with 968f-GC/1401r – the index was higher though the changes were proportional for both cases. The results were confirmed with genetic similarity analysis presented as dendrograms.

Słowa kluczowe

EN

PCR-DGGE; SBR; bacterial community monitoring; reject water

PL

odcieki; SBR; PCR-DGGE

• Wydawca: Instytut Podstawowych Problemów Techniki PAN
• Czasopismo: Engineering Transactions
• Rocznik: 2014
• Tom: Vol. 62, iss. 2
• Strony: 95--107
• Opis fizyczny: Bibliogr. 15 poz., rys., tab., wykr.

Twórcy

autor

Ziembińska-Buczyńska A.

• Silesian University of Technology, Environmental Biotechnology Department Akademicka 2, 44-100 Gliwice

autor

Cema G.

• Silesian University of Technology, Environmental Biotechnology Department Akademicka 2, 44-100 Gliwice

autor

Szpindor M.

• Silesian University of Technology, Environmental Biotechnology Department Akademicka 2, 44-100 Gliwice

autor

Meresta A.

• Silesian University of Technology, Environmental Biotechnology Department Akademicka 2, 44-100 Gliwice

autor

Płonka L.

• Silesian University of Technology, Environmental Biotechnology Department Akademicka 2, 44-100 Gliwice

Bibliografia

• 1. Arnaud M., Bernier L., Filion M., Hamelin R., Molecular profiling of rhisosphere microbial communities associated with healthy and diseased black spruce (Picea mariana) seedlings grown in a nursery, Applied and Environmental Microbiology, 70, 6, 3541–3551, 2004.
• 2. Cema G., Schneider Y., Beier M., Rosenwinkel K-H., Influence of Free Ammonia and Free Nitrous Acid on Anammox Activity, in: Proceeding of: WEF/IWA Nutrient Removal and Recovery, Trends in Resources Recovery and Use, At Vancouver, 2013.
• 3. Dapena-Mora A., Fernandez I., Campos J.L., Mosquera-Corral A., M´endez R., Jetten M.S.M., Evaluation of Activity and Inhibition effects on Anammox process by batch tests based on nitrogen gas production, Enzyme and Microbial Technology, 40, 4, 859–865, 2007.
• 4. Dell’Abate M., Insam H., Klammer S., Knapp B., Ros M., Bacterial community patterns and thermal analyses of composts of various origins, Waste Management & Research, 26, 173–187, 2008.
• 5. Felske A., Engelen B., Bel U.N., Backhaus H., Direct ribosome isolation from soil to extract bacterial rRNA for community analysis, Applied and Environmental Microbiology, 62, 11, 4162–4167, 1996.
• 6. Fernandez I., Dosta J., Fajardo C., Campos J.L., Mosquera-Coral A., Mende R., Short-and long-term effects of ammonium and nitrite on the Anammox process, Journal of Environmental Management, 95, 170–174, 2012.
• 7. Gao F., Jiang Y., Xu X., Ye K., Zhou G., Changes in the composition of the bacterial flora on tray-packaged pork during chilled storage analyzed by PCR-DGGE and real-time PCR, Journal of Food Science, 76, 27–33, 2011.
• 8. Jaroszynski L., Cicek N., Sparling R., Oleszkiewicz J., Impact of free ammonia on anammox rates (anoxic oxidation) in a moving bed biofilm reactor, Chemosphere, 88, 2, 188–195, 2012.
• 9. Lotti T., van der Star W.R.L., Kleerebezem R., Lubello C., van Loosdrecht M.C.M., The effect of nitrite inhibition on the anammox process, Water Research, 46, 8, 2559–2569, 2012.
• 10. Muyzer G., De Waal E.C., Uitierlinden A.G., Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S rRNA, Applied and Environmental Microbiology, 59, 695– 700, 1993.
• 11. Nubel U., Engelen B., Felske A., Snaidr J., Wieshuber A., Amann R.I., Lud- ¨ wig W., Backhaus H., Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis, Journal of Bacteriology, 178, 5636–43, 1996.
• 12. Ryzińska J., Problem wód osadowych i możliwości ich oczyszczania w Polsce, Woda i Technika Sanitarna, 7–8, 58–62, 2006.PCR-DGGE BASED BIODIVERISTY, CHANGEABILITY AND GENETIC. . . 107
• 13. Strous M., Kuenen J.G., Jetten M.S.M., Key physiology of anaerobic ammonium oxidizing microorganism, Applied Microbiology and Biotechnology, 65, 3248–3250, 1999.
• 14. Ziembińska A., Ciesieski S., Miksch K., Ammonia oxidizing bacteria community in activated sludge monitored by denaturing gradient gel electrophoresis (DGGE), Journal of General and Applied Microbiology, 55, 375–380, 2009.
• 15. Ziembinska A., Ciesielski S., Wiszniowski J., Advantages and disadvantages of nested PCR-DGGE approach for ammonia oxidizers monitoring in membrane bioreactor activated sludge, in print: Archives of Environmental Protection, 4, 2014.