Application of foam separation in production of β-glucanase in Pichia

• Autorzy: Yuhang Qin , Yuqing Chen , Yajuan Peng , Wenyao Shao

Identyfikatory

DOI

10.2478/pjct-2022-0015

• Języki publikacji: EN

Abstrakty

EN

β-glucanase is widely used in many fields and has great economic value and development space, but it faces the difficulties of separation and nutrient destruction in the process of industrial production. Foam separation is a simple, mild and efficient adsorption separation technique that enables efficient separation and extraction of β-glucanase. In this study, five single factors(loading volume, pH, separation gas velocity, fermentation loading concentration, surfactant concentration) of foam separation and harvest of β-glucanase produced by Pichia pastoris were studied. The best univariate condition was: 600 mL/min separation gas velocity, loading volume of 200 mL, initial enzyme concentration of 100 g/mL, surfactant concentration of 0.3 mg/mL and pH of 5. Based on the best univariate condition, the optimal separation conditions of β-glucanase were further explored, and the five-factor four-level orthogonal test was designed. From the experimental results, the best separation condition was: 600 mL/min, loading volume of 200 mL, initial enzyme concentration of 100 μg/mL, surfactant concentration of 0.5 mg/mL and pH of 5. Under this separation condition, the enrichment ratio (E) was 0.56 and the recovery rate (R) was 96.01%.

Słowa kluczowe

EN

Foam separation; β-glucanase; recovery rate; enrichment ratio

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2022
• Tom: Vol. 24, nr 3
• Strony: 1--7
• Opis fizyczny: Bibliogr. 22 poz., rys., tab., wz.

Twórcy

autor

Yuhang Qin

• College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, P.R. China

autor

Yuqing Chen

• College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, P.R. China

autor

Yajuan Peng

• College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, P.R. China

autor

Wenyao Shao

• College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, P.R. China

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).