Characterization of novel trypsin inhibitor in raw and toasted peanuts using a simple improved isolation

• Autorzy: Medeiros Amanda Fernandes , Rocha Maria Gabriela Ferreira , Serquiz Alexandre Coelho , Machado Richele Janaína Araújo , Lima Vanessa Cristina Oliveira , Carvalho Fabiana Maria Coimbra , Costa Izael Sousa , Maciel Bruna Leal Lima , Santos Elizeu Antunes , Morais Araújo Ana Heloneida

Identyfikatory

DOI

10.1556/1326.2017.00353

• Języki publikacji: EN

Abstrakty

EN

Trypsin inhibitors have been described in peanuts and their derived industrialized foods, demonstrating diversity and thermoresistance. Given their most varied applications, these enzymatic protease inhibitors have been isolated and characterized for their potential use as bioinsecticides, herbal medicines, or medicines, but it is not simple. There are still no reports in the literature of the isolation and characterization of trypsin inhibitors in cultivar cavalo rosa (CCR) peanut, a common variety in Brazil. However, there are biological activities related to trypsin inhibitors from peanut-derived products. In this study, we isolated and characterized a novel trypsin inhibitor in CCR peanuts (Arachis hypogaea L.) under different processing conditions using a simple improved isolation. Raw and toasted peanut inhibitor was isolated by ammonium sulfate fractionation and trypsin-cyanogen bromide-activated Sepharose® 4B (CNBr-Sepharose® 4B) chromatography. The inhibitors from raw and toasted peanut were called AhTI1 and AhTI2, respectively, with potent anti-trypsin activity. Activity at different temperatures and pH was evaluated, and both samples were similarly stable under tested conditions. Minimum concentration for inhibition to occur (IC50) was 2.78 × 10^-10 M and 2.39 × 10^-10 M for AhTI1 and AhTI2, and inhibition constant (Ki) was 3.26 × 10−10 M and 1.54 × 10−10 M, respectively, showing non-competitive reversible kinetics. We concluded that AhTI1 and AhTI2 presented highly specific to trypsin and stable to toasting, different temperatures, and pH ranging. These are important characteristics in the process of developing bioinsecticides or biopharmaceuticals. Thus, this may be an interesting molecule, aiming at its biotechnological application, and it was obtained using a simple and easy isolation process.

Słowa kluczowe

EN

Arachis hypogaea L; protein inhibitors; anti-trypsin activity; chromatography thermoresistance; bioactive protein

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 2
• Strony: 79--84
• Opis fizyczny: Bibliogr. 47 poz., rys., tab.

Twórcy

autor

Medeiros Amanda Fernandes

• Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Rocha Maria Gabriela Ferreira

• Course of Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Serquiz Alexandre Coelho

• Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Machado Richele Janaína Araújo

• Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Lima Vanessa Cristina Oliveira

• Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Carvalho Fabiana Maria Coimbra

• Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Costa Izael Sousa

• Department of Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil
• Postgraduate Nutrition Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Maciel Bruna Leal Lima

• Course of Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil
• Postgraduate Nutrition Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Santos Elizeu Antunes

• Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil
• Department of Biochemistry, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil

autor

Morais Araújo Ana Heloneida

• Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil
• Department of Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil
• Postgraduate Nutrition Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).