Radicals initiated by gamma rays in selected amino acids and collagen

• Autorzy: Przybytniak Grażyna. , Sadło Jarosław , Dąbrowska Małgorzata , Zimek Zbigniew

Identyfikatory

DOI

10.2478/nuka-2019-0002

• Języki publikacji: EN

Abstrakty

EN

Calf skin collagen and three amino acids essential for its structure, namely glycine, L-proline and 4-hydroxyl-L-proline, were irradiated with gamma rays up to a dose of 10 kGy. Conversion of radicals over time or after thermal annealing to selected temperatures was monitored by X-band electron paramagnetic resonance (EPR) spectroscopy. Some experimental spectra were compared with signals simulated based on literature data from the electron nuclear double resonance (ENDOR) studies. The following phenomena were confi rmed in the tested amino acids: abstraction of hydrogen atom (glycine, proline, hydroxyproline, collagen), deamination (glycine, hydroxyproline), decarboxylation (hydroxyproline). Chain scission at glycine residues, radiation-induced decomposition of side groups and oxidative degradation were observed in irradiated collagen. The decay of radicals in collagen saturated with water occurred at lower temperatures than in macromolecules having only structural water. The paramagnetic centres were the most stable in an oxygen-free atmosphere (vacuum). Radical processes deteriorated the structure of collagen; hence, radiation sterilization of skin grafts requires careful pros and cons analysis.

Słowa kluczowe

EN

amino acid; collagen; EPR spectroscopy; gamma rays

PL

aminokwas; kolagen; spektroskopia EPR; promienie gamma

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2019
• Tom: Vol. 64, No. 1
• Strony: 11--17
• Opis fizyczny: Bibliogr. 28 poz., rys.

Twórcy

autor

Przybytniak Grażyna.

• Institute of Nuclear Chemistry and Technology 16 Dorodna St., 03-195 Warsaw, Poland

autor

Sadło Jarosław

• Institute of Nuclear Chemistry and Technology 16 Dorodna St., 03-195 Warsaw, Poland

autor

Dąbrowska Małgorzata

• Institute of Nuclear Chemistry and Technology 16 Dorodna St., 03-195 Warsaw, Poland

autor

Zimek Zbigniew

• Institute of Nuclear Chemistry and Technology 16 Dorodna St., 03-195 Warsaw, Poland

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).