Results of analyses of airborne particulates collected in winter and summer seasons from two locations of Wroclaw (city centre and the outskirts area) have been presented. Samples of particulate matter were collected on sintered-glass filters, using a high performance air aspirator Staplex-PMIO. Extraction with dichloromethane was carried out in a Soxhlet apparatus. Mutagenicity of dichloromethane extracts was studied based on a conventional Salmonella assay. Two Salmonella typhimurium strains TA98 and YG1041 were employed in the assay. Genotoxicity of organic air pollutants was detected using a miniaturized SOS chromotest obtained from EBPI (Brampton, Ontario, Canada). Bacterial assays were carried out with and without metabolic activation by the S9 microsomal fraction. Cytotoxicity of the particulate extracts was studied by the method of their direct contact with onelayer culture of human lung carcinoma epithelial cells - A549.
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Omówiono ważniejsze ustawy i rozporządzenia dotyczące powołania w Polsce organizacji do walki z zanieczyszczeniami środowiska morskiego oraz realizację tego przedsięwzięcia. Przeanalizowano sposób prowadzenia kontroli powierzonego obszaru morza oraz sporządzania dokumentacji zdarzenia.
EN
The paper discusses more important laws and regulations concerning the establishment in Poland of an organization for combating the marine environment pollution and the realization of such an endeavor. Discussed are also methods of conducting a proper surveillance over an entrusted sea territory and preparation of the documentation of the case.
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