The effect of selected plant extracts on the mitochondria of leukemic cancer cells in vitro

• Autorzy: Tomečková V. , Stupák M. , Mojžišová G. , Šutorová M. , Mašlanková J.

Warianty tytułu

PL

Wpływ wybranych wyciągów roślinnych na mitochondria białaczkowych komórek nowotworowych in vitro

• Języki publikacji: EN

Abstrakty

EN

Individual plant extracts from horse chestnut, arnica and hops have been applied in vitro on Jurkat type leukemic cells from which mitochondria were isolated. Each sample of the isolated experimental mitochondria were monitored by the determination of proteins and by monitoring the fluorescence of endogenous fluorophores of experimental mitochondria compared with control mitochondria isolated from intact leukemic cells by means of the synchronous fluorescence fingerprint method. The horizontal cut of excitation-emission matrices at (λ = 240 nm) demonstrated the final graphical and mathematical fluorescence of mitochondria. The result is the presence of two fluorescent excitation-emission zones (the result of the overlapping effect of very similar fluorescence maxima values), which were subsequently compared with standard fluorophores. A reduction of protein content in all experimental mitochondria was observed, the most after the effect of arnica and the least after the influence of horse chestnut. The decrease of fluorescence of the first fluorescent zone was compared to control mitochondria. The decline of mitochondrial fluorescence confirmed the cytotoxic effect of the substances studied on the mitochondria in vitro.

PL

Oceniono wpływ in vitro wyciągów wybranych roślin (kasztanowiec, chmiel, arnica) na mitochondria wyizolowane z nowotworowych komórek białaczkowych typu Jurkat. Każdą próbkę wyizolowanych eksperymentalnie mitochondriów monitorowano określając produkowane białka i oceniając fluorescencję endogennych fluoroforów z użyciem metody the synchronous fluorescence fingerprint i porównano z mitochondriami kontrolnymi uzyskanymi z komórek nie poddanych oddziaływaniu wyciągów. Odczyty wskazań emisji macierzy falą o długości (λ = 240 nm) ukazały fluorescencję mitochondriów. Stwierdzono dwie emisje, które porównano ze standardowymi fluorophorami. Zobrazowano redukcję zawartości białka we wszystkich doświadczalnych mitochondriach, największą po wyciągach z arnica i najmniejszą po kasztanowcu. Obniżenie fluorescencji pierwszej strefy było porównywalne z mitochondriami kontrolnymi. Obniżenie mitochondrialnej fluorescencji potwierdza cytotoksyczny efekt badanych substancji na mitochondia in vitro.

Słowa kluczowe

EN

synchronous fluorescence fingerprint; fluorescence; mitochondria; arnika; hops; horse chestnut; cytotoxic effect; leukemic cells

PL

synchronous fluorescence fingerprint; fluorescencja; mitochondria; arnica; chmiel; kasztanowiec; efekt cytotoksyczny; białaczkowe komórki nowotworowe

• Wydawca: Górnośląska Wyższa Szkoła Pedagogiczna im. Kardynała Augusta Hlonda
• Czasopismo: Journal of Ecology and Health
• Rocznik: 2013
• Tom: R. 17, nr 4
• Strony: 186--190
• Opis fizyczny: Bibliogr. 22 poz., rys., wykr.

Twórcy

autor

Tomečková V.

• Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine UPJŠ, Trieda SNP 1, 040 66 Košice, Slovakia

autor

Stupák M.

• Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine UPJŠ, Trieda SNP 1, 040 66 Košice, Slovakia

autor

Mojžišová G.

• Department of Experimental Medicine, Faculty of Medicine, UPJŠ, Trieda SNP 1, 040 66 Košice, Slovakia

autor

Šutorová M.

• Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine UPJŠ, Trieda SNP 1, 040 66 Košice, Slovakia

autor

Mašlanková J.

• Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine UPJŠ, Trieda SNP 1, 040 66 Košice, Slovakia

Bibliografia

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