Validated LC-MS/MS method for quantitation of solasodine in rat urine and feces : blocking nonspecific adsorption

• Autorzy: Lu Tiantian , Wang Xiaohong , Zhang Qi , Liu Kun , Xu Tongxin , Wang Quande , Zhao Pengfei , Cheng Zhongzhe

Identyfikatory

DOI

10.1556/1326.2022.01079

• Języki publikacji: EN

Abstrakty

EN

Solasodine, a steroidal alkaloid, is distributed extensively in Solanaceae plants with multiple biological activities such as neuroprotection, antineoplastic and anticonvulsant activities. However, there is little information about the excretion of intact solasodine in vivo. To investigate its excretion, a reliable LC-MS/MS method for quantitation solasodine in rat urine and feces was established and validated. Sample preparation was carried out by liquid-liquid extraction using MTBE as extractant. Moreover, rat urine was preconditioned with BSA, an anti-adsorptive additive, to prevent the nonspecific binding of solasodine to containers and tubes. The method was validated over the range of 4–2000 ng mL⁻¹. The correlation coefficient (r^2t) were all above 0.999. The intra- and inter-day precision and accuracy were within 16.9% and between −11.0 and 8.9%, respectively. The recovery of solasodine in urine and feces was in the range of 72.5–80.3 and 75.7–80.2%, respectively. IS-normalized matrix factor ranged from 0.94 to 1.12 with RSD% ≤4.02%. This method was successfully applied to the excretion study of solasodine following oral and intravenous administration.

Słowa kluczowe

EN

solasodine; excretion; anti-adsorption; LC-MS/MS

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 4
• Strony: 319--325
• Opis fizyczny: Bibliogr. 17 poz., rys., tab., wykr.

Twórcy

autor

Lu Tiantian

• School of Pharmacy, Weifang Medical University, 7166 Baotong West Street, Weifang, Shandong 261053, China

autor

Wang Xiaohong

• School of Pharmacy, Weifang Medical University, 7166 Baotong West Street, Weifang, Shandong 261053, China

autor

Zhang Qi

• School of Pharmacy, Weifang Medical University, 7166 Baotong West Street, Weifang, Shandong 261053, China

autor

Liu Kun

• School of Pharmacy, Weifang Medical University, 7166 Baotong West Street, Weifang, Shandong 261053, China

autor

Xu Tongxin

• School of Pharmacy, Weifang Medical University, 7166 Baotong West Street, Weifang, Shandong 261053, China

autor

Wang Quande

• State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, 15 Yucai Road, Qixing District, Guilin, Guangxi 541004, China

autor

Zhao Pengfei

• Department of Clinical Pharmacy, Weifang People’s Hospital, 151 Guangwen Street, Kuiwen District, 261031, Weifang, Shandong 261053, China

autor

Cheng Zhongzhe

• School of Pharmacy, Weifang Medical University, 7166 Baotong West Street, Weifang, Shandong 261053, China

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