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Abstrakty
A simple and reliable TLC method for analysis of -lipoic acid (LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm × 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6:4:2:8:0.2 ( v/v ) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0–3.0 µg per band) and second-degree polynomial (0.5–5.0 µg per band) regression functions. The correlation coefficient ( r = 0.999), the limit of quantification (0.39 µg per band), recovery (98.5–105.2%), and precision (1.8–2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5–101.0% in the drug formulations, 98.8–99.5% in three of five dietary supplements tested, and 48.0–185.0% in two other dietary supplements.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
433--441
Opis fizyczny
Bibliogr. 26 poz., rys., tab.
Twórcy
autor
- University of Belgrade Faculty of Pharmacy Serbia Yugoslavia
autor
- University of Belgrade Faculty of Pharmacy Serbia Yugoslavia
autor
- University of Belgrade Faculty of Pharmacy Serbia Yugoslavia
autor
- University of Belgrade Faculty of Pharmacy Serbia Yugoslavia
autor
- National Institute of Chemistry Laboratory for Food Chemistry Ljubljana Slovenia
autor
- National Institute of Chemistry Laboratory for Food Chemistry Ljubljana Slovenia
autor
- University of Belgrade Faculty of Pharmacy Serbia Yugoslavia
Bibliografia
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- [6] D.R. Tomlinson, Diabetes Metab., 24 , 79 (1998)
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- [14] J. Chen, W. Jiang, J. Cai, W. Tao, X. Gao, and X. Jiang, J. Chromatogr. B, 824 , 249 (2005)
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- [17] M.N. Abbas and A.A. Radwan, Talanta, 74 , 1113 (2008)
- [18] A. Sitton, M.G. Schmid, G. Gubitz, and H.Y. Aboul-Enein, J. Biochem. Biophys. Methods, 61 , 119 (2004)
- [19] S. Satoh, T. Toyo’oka, T. Fukushima, and S. Inagaki, J. Chromatogr. B, 854 , 109 (2007)
- [20] A.I. Durrani, H. Schwartz, W. Schmid, and G. Sontag, J. Pharm. Biomed. Anal., 45 , 694 (2007)
- [21] O. Corduneanu, M. Garnett, and A.M.O. Bret, Anal. Lett., 40 , 1763 (2007)
- [22] S.D. Wagner, S.W. Kaufer, and J. Sherma, J. Liq. Chromatogr. Related Technol., 24 , 2525 (2001)
- [23] European Pharmacopoeia, 6th edn, EDQM, Council of Europe, Strasbourg, France, 2008, 3055, 461
- [24] H. Jork, W. Funk, W. Fischer, and H. Wimmer, Thin-Layer Chromatography: Reagents and Detection Methods, VCH, Weinheim, Germany, 1994, 338
- [25] Z. Korićanac, M. Čakar, S. Tanasković, and T. Jovanović, J. Serb. Chem. Soc., 72 , 29 (2007)
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Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-fcaecf3f-c9e7-44e2-8311-051c8f1ff18d