Analysis of α -lipoic acid in drug formulations and dietary supplement preparations

• Autorzy: Ravanić N , Filipić S. , Nikolić K , Popović G , Vovk I , Simonovska B , Agbaba D
• Języki publikacji: EN

Abstrakty

EN

A simple and reliable TLC method for analysis of -lipoic acid (LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm × 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6:4:2:8:0.2 ( v/v ) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0–3.0 µg per band) and second-degree polynomial (0.5–5.0 µg per band) regression functions. The correlation coefficient ( r = 0.999), the limit of quantification (0.39 µg per band), recovery (98.5–105.2%), and precision (1.8–2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5–101.0% in the drug formulations, 98.8–99.5% in three of five dietary supplements tested, and 48.0–185.0% in two other dietary supplements.

Słowa kluczowe

EN

α-Lipoic acid; thin-layer chromatography; densitometry; dietary supplements

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2009
• Tom: Vol. 21, no. 3
• Strony: 433--441
• Opis fizyczny: Bibliogr. 26 poz., rys., tab.

Twórcy

autor

Ravanić N

• University of Belgrade Faculty of Pharmacy Serbia Yugoslavia

autor

Filipić S.

• University of Belgrade Faculty of Pharmacy Serbia Yugoslavia

autor

Nikolić K

• University of Belgrade Faculty of Pharmacy Serbia Yugoslavia

autor

Popović G

• University of Belgrade Faculty of Pharmacy Serbia Yugoslavia

autor

Vovk I

• National Institute of Chemistry Laboratory for Food Chemistry Ljubljana Slovenia

autor

Simonovska B

• National Institute of Chemistry Laboratory for Food Chemistry Ljubljana Slovenia

autor

Agbaba D

• University of Belgrade Faculty of Pharmacy Serbia Yugoslavia

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