Sensitivity of Fusarium solani isolated from asparagus on essential oils

• Autorzy: Grata K.

Identyfikatory

DOI

10.2428/ecea.2016.23(4)32

Warianty tytułu

PL

Wrażliwość Fusarium solani wyizolowanego ze szparaga na olejki eteryczne

• Języki publikacji: EN

Abstrakty

EN

The purpose of this study was to evaluate the antifungal properties of sage oil, clove oil and basil oil against Fusarium solani isolated from white asparagus spears (Asparagus officinalis L.). A dual culture plate method was used for the assessment of the inhibitory effects of essential oils and volatile components on mycelium, inoculated into a PDA medium. The culturing process was conducted for 9 days at a temperature of 26^oC and the fungal linear growth was measured every 1–3 days. The conidial germination of the fungus in the presence of oils was evaluated by microscope method. The results show differences in the fungistatic activity of oils, dependent on the type and dose of the oil. Generally the oils had a higher impact against the fungal spores than on mycelium growth of F. solani, however the type of oil and dose determined different amounts. The conidial germination was most inhibited by the basil oil (98.9% maximum inhibition), followed by the clove and sage oils at a concentration of 4% (respectively 89.9% and 85.4% maximum inhibition). In contrast, the oils had much less of an impact on the linear mycelial growth of F. solani. Use of the highest dose of clove oil reduced the linear growth of mycelium by 29.9%, sage oil by 14.8% and basil oil by 4%. Furthermore, gas metabolites limited the growth of F. solani. The degree of inhibition of mycelium amounted to 17.1% for clove oil, 15.5% for sage oil and 9.3% for basil oil used in the maximum concentration 4.0%. Based on these results, clove oil was the greatest inhibitor of mycelial growth and sporulation of F. solani.

PL

Celem badań była ocena przeciwgrzybicznych właściwości olejku z szałwii, olejku goździkowego i olejku bazyliowego wobec Fusarium solani wyizolowanego z pędów szparaga (Asparagus officinalis L.) Ocenę właściwości antagonistycznych testowanych olejków i ich metabolitów gazowych wobec grzybni przeprowadzono metodą hodowlano-płytkową na podłożu PDA. Hodowlę prowadzono przez 9 dni w temp. 26^oC, dokonując pomiarów co 1–3 dni. Natomiast zarodnikowanie grzyba w obecności olejków oceniono metodą mikroskopową. Przeprowadzone badania wykazały różnice w aktywności metabolicznej testowanych olejków. W zależności od rodzaju olejku i jego dawki, stwierdzono większą aktywność wobec zarodników grzybów niż na wzrost grzybni F. solani. Największy wpływ na zdolność kiełkowania zarodników wykazał olejek bazyliowy, następnie kolejno olejek goździkowy i olejek szałwiowy w stężeniu 4%. Stopień zahamowania kiełkowania zarodników wyniósł odpowiednio: 98,9%, 89,9% oraz 85,4%. Natomiast zdecydowanie mniejszy wpływ wykazały na wzrost grzybni F. solani. Zastosowanie największej dawki olejku goździkowego ograniczy ło tempo wzrostu grzyba o 29,9%, olejku szałwiowego o 14,8% a olejku bazyliowego tylko o 4,0%. Równie ż metabolity gazowe ograniczały wzrost grzybni F. solani. Stopień zahamowania wzrostu wynosił odpowiednio 17,1% dla olejku goździkowego, 15,5% dla olejku szałwiowego i 9,3% dla bazyliowego. Biorąc pod uwagę analizowane czynniki, olejek goździkowy okazał się największym inhibitorem kiełkowania zarodników i wzrostu grzybni F. solani.

Słowa kluczowe

EN

Fusarium solani; asparagus; essential oils; antifungal activity

PL

Fusarium solani; szparagi; olejki eteryczne; aktywność przeciwgrzybowa

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. A
• Rocznik: 2016
• Tom: Vol. 23, nr 4
• Strony: 453--464
• Opis fizyczny: Bibliogr. 62 poz., tab., wykr.

Twórcy

autor

Grata K.

• Independent Chair of Biotechnology and Molecular Biology, University of Opole, ul. kard. B. Kominka 6, 45–035 Opole, Poland, phone: [phone]

Bibliografia

• [1] Stępień Ł, Waśkiewicz A, Urbaniak M. Wildly growing asparagus (Asparagus officinalis L.) hosts pathogenic Fusarium species and accumulates their mycotoxins. Microb Ecol. 2016;71:927-937. DOI: 10.1007/s00248-015-0717-1.
• [2] Wang J, Wang X, Zhou Y, Du L, Wan Q. Fumonisin detection and analysis of potential fumonisinproducing Fusarium spp. in asparagus (Asparagus officinalis L.) in Zhejiang Province of China. J Sci Food Agric. 2010;90:836-842. DOI: 10.1002/jsfa.3893.
• [3] Weber Z, Kostecki M, Bargen S, Gossmann M, Waśkiewicz A, Bocianowski J, Knaflewski M, et al. Fusarium species colonizing spears and forming mycotoxins in field samples of asparagus from Germany and Poland. J Phytopathol. 2006;154:209-2016. DOI: 10.1111/j.1439-0434.2006.01085.x.
• [4] Waśkiewicz A, Irzykowska L, Bocianowski J, Karolewski Z, Kostecki M, Weber Z, Goliński P. Occurence of fusarium and mycotoxins in marketable asparagus spears, Pol J Environ Stud. 2010;19(1):219-225.
• [5] Waśkiewicz A, Irzykowska L, Drzewiecka K, Bocianowski J, Dobosz B, Weber Z, Karolewski Z, et al. Plant-pathogen interactions during infection process of asparagus with Fusarium spp. Cent Eur J Biol. 2013;8(11):1065-1076. DOI: 10.2478s11535-013-0217-6.
• [6] Kalomira E. Asparagus diseases. EJPSB. 2007;1(1):76-83.
• [7] Grata K. Aktywność biologiczna Bacillus amyloliquefaciens wobec Fusarium spp. wyizolowanych z pędów szparaga (Asparagus officinalis L.) [Biocontrol activities of Bacillus amyloliquefaciens against Fusarium spp. isolated from the white spears of asparagus (Asparagus officinalis L.)]. 2016:7-22. In: Mikrobiologia oraz metody analityczne w nauce [Microbiology and analytical methods in science]. Kropiwiec K, Szala M, editors, Lublin: TYGIEL Publishers, ISBN 978-83-65598-27-1.
• [8] Borrego-Benjumea A, Basallote-Ureba M J, Melero-Vara J, M, Pervaiz A. Characterization of Fusarium isolates from asparagus fields in southwestern Ontario and influence of soil organic amendments on Fusarium crown and root rot. Phytopathology. 2014;104(4):403-415. DOI: 10.1094/PHYTO-08-13-0231-R.
• [9] Waśkiewicz A, Goliński P, Karolewski Z, Irzykowska L, Bocianowski J, Kostecki M, Weber Z. Formation of fumonisins and other secondary metabolites by Fusarium oxysporum and F. proliferatum: a comparative study. Food Add Contam. 2010;27:608-615. DOI: 10.1080/19440040903551947.
• [10] Weber Z, Kostecki M, von Bargen S, Gossmann M, Waśkiewicz A, Bocianowski J, Kaflewski M, et al. Fusarium species colonizing spears and forming mycotoxins in field samples of asparagus from Germany and Poland. J Phytopathol. 2006;154;209-216. DOI: /10.1111/j.1439-0434.2006.01085.x
• [11] Nahiyan ASM, Robinson BL, Jeffries P, Matsubara Y. PCR-SSCP analysis of Fusarium diversity in asparagus decline in Japan. Eur J Plant Pathol. 2011;130:197-203. DOI: 10.1007/s10658-011-9745-y.
• [12] Andrzejak R, Werner M. Grzyby z rodzaju Fusarium, uszkadzające wypustki szparagów i ich patogeniczność względem młodych roślin [Fungi form genus Fusarium spp. damaging asparagus spears and their pathogenicity towards young plants]. Prog Plant Prot. 2006;46(2):700-703.
• [13] Weber Z, Karolewski L, Irzykowska L, Knaflewski M, Kosiada T. Occurrence of Fusarium species in spears of asparagus (Asparagus officinalis). Phytopathol Pol. 2007;45:9-15.
• [14] Elmer W. H. Management of Fusarium crown and root rot of asparagus. Crop Prot. 2013;73:2-6. DOI: 10.1016/j.cropro.2014.12.005.
• [15] Qiu M, Wu Ch, Ren G, Liang X, Wang X, Huang J. Effect of chitosan and its derivatives as antifungal and preservative agents on postharvest green asparagus. Food Chem. 2014;155:105-111. DOI: 10.1016/j.foodchem.2014.01.026.
• [16] Rubio-Perez DE, Molinero-Ruiz ML, Melero-Vara JM, Basalote-Ureba MJ. Selection of potential antagonists against asparagus crown and root rot caused by Fusarium spp. Comm Appl Biol Sci. 2008; 3(2):203-204.
• [17] Yergeau E, Sommerville DW, Maheux E, Vujanovic V, Hamel Ch, Whalen JK, St-Arnaud M. Relationships between Fusarium population structure, soil nutrient status and disease incidence in field-grown asparagus. FEMS Microbiol Ecol. 2006;58:394-403. DOI: 10.1111/j.1574-6941.2006.00161.x.
• [18] Borrego-Benjumea AI, Melero-Vara JM, Basallote-Ureba MJ. Organic amendments conditions on the control of Fusarium crown and root rot of asparagus caused by three Fusarium spp. Spain J Agric Res. 2015;13(4):1-13. DOI: 10.5424/sjar/2015134-7905.
• [19] Arriola LL, Hausbeck MK, Rogers J, Safir GR. The effect of Trichoderma harzianum and arbuscular mycorrhizae on Fusarium root rot in Asparagus. HorTechnology. 2000;10(1):141-144.
• [20] Manganyi MC, Regnier T, Olivier EJ. Antimicrobial activities of selected essential oils against Fusarium oxysporium isolates and their biofilms. SAFR J Bot. 2015;99:115-121. DOI: 10.1016/j.sajb.2015.03.192.
• [21] Elgorban AM, Bahkali AH, El-Metwally MA, Elsheshtawi M, Abdel-Wahab MA. In vitro antifungal activity of some plant essential oils. Int J Pharmcol. 2015;11(1):56-61. DOI: 10.3923/ijp.2015.56.61.
• [22] Naeini A, Ziglari T, Shokri H., Khosravi AR. Assessment of growth-inhibiting effect of some plant essential oils on different Fusarium isolates. J Mycol Med. 210;20:174-178. DOI: 10.1016/j.mycmed.2010.05.005.
• [23] Snieškienè V, Stankevičienè A, Varkulevičienè J. The effect of the essential oils on micromycetes isolated from plants. Zemdirbyste-Agriculture. 2008;95(3):447-452.
• [24] Yaouba A, Tatsadjieu NL, Jazet DPM, Etoa FX, Moses MC. Antifungal properties of essential oils and some constituents to reduce foodborne pathogen. J Yeast Fungal Res. 2010;1(1):001-008.
• [25] Tabassum N, Vidyasagar GM. Antifungal investigations on plant essential oils. A review. Int J Pharm Pharm Sci. 2013;5(2):19-28.
• [26] Malkhan SG, Shahid A, Masood A, Kangabam SS. Efficacy of plant extracts in plant disease management. Agr Sci. 2012;3:425-433. DOI: 10.4236/as.2012.33050.
• [27] Sameza ML, Mabou LCN, Tchameni SN, Bedine MAB, Tchoumbougnang F, Dongmo PMJ, Fekam FB. Evaluation of clove essential oil as a mycobiocide against Rhizopus stolonifer and Fusarium solani, Tuber Rot Causing Fungi in Yam (Dioscorea rotundata Poir.). J Phytopathol. 2016;164:433-440. DOI: 10.1111/jph.12468.
• [28] Antunes MDC, Cavaco AM. The use of essential oils for postharvest decay control. A review. Flavour Fragr. 2010;25:351-366. DOI: 10.1002/ffj.1986.
• [29] Kumar V, Mathela CS, Tewari AK, Bisht KS. In vitro inhibition activity of essential oils from some Lamiaceae species against phytopathogenic fungi. Pestic Biochem Phys. 2014;114:67-71. DOI: 10.1016/j.pestbp.2014.07.001.
• [30] http://mazidła.com
• [31] Gömöri C, Nacsa-Farkas E, Kerekes EB, Kocsubé S, Vágvölgyi C, Krisch J. Evaluation of five essential oils for the control of foodspoilage and mycotoxin producing fungi. Acta Biol Szeged. 2013;57(2):113-116.
• [32] Tyagi AK, Malik A. Antimicrobial potential and chemical composition of Mentha piperita oil in liquid and vapour phase against food spoiling microorganisms. Food Control. 2011;22:1707-1714. DOI: 10.1016/j.foodcont.2011.04.002.
• [33] Burgieł Z. Wpływ niektórych herbicydów na występowanie i rozwój patogenów powodujących choroby podsuszkowe pszenicy ozimej. [Effect of some herbicides on the incidence and growth of pathogens causing wheat eyespot]. Cz. II. Rozwój patogenów [Development of pathogens.]. Acta Agr Silv Seria Agraria. 1984;23:187-196.
• [34] Burgieł Z, Kończyk D. Reakcje grzyba Fusarium culmorum wyizolowanego z korzeni pszenicy ozimej na wybrane herbicydy. [The reactions of the fungus Fusarium culmorum isolated from the roots of winter wheat to selected herbicides]. Acta Agraria et Silvestria 1989;XXVIII:25-31.
• [35] Hussain AI, Anwar F, Sherazi STH , Przybylski R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 2008;108:986-995. DOI: 10.1016/j.foodchem.2007.12.010.
• [36] Daferera DJ, Ziogas BN, Polissiou MG. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. Michiganensis Crop Prot. 2003;22:39-44.
• [37] Abu-Darwish MS, Cabral C, Ferreira IV, Gonçalves MJ, Cavaleiro C, Cruz MT, Al-Bdour TH, Salgueiro L. Essential Oil of Common Sage (Salvia officinalis L.) from Jordan: Assessment of safety in mammalian cells and its antifungal and anti-inflammatory potential. BioMed Res Int. 2013; Article ID 538940: 9 pages. DOI: 10.1155/2013/538940.
• [38] Farhat MB, Jordán MJ, Chaouech-Hamada R, Landoulsi A, Sotomayor JA. Variations in essential oil, phenolic compounds, and antioxidant activity of tunisian cultivated Salvia officinalis L. J Agr Food Chem. 2009;57(21):10349-10356. DOI 10.1021/jf901877x.
• [39] Gupta A, Sharma S, Naik SN. Biopesticidal value of selected essential oils against pathogenic fungus, termites, and nematodes. Int Biodeter Biodegr. 2011;65:703-707. DOI: 10.1016/j.ibiod.2010.11.018.
• [40] Pitarokili D, Couladis M, Petsikos-Panayotarou N, Tzakou O. Composition and Antifungal Activity on Soil-Borne Pathogens of the Essential Oil of Salvia sclarea from Greece. J Agric Food Chem. 2002;509(23): 6688-6691. DOI: 10.1021/ jf020422n.
• [41] Džamić A, Soković M, Ristić M, Grujić-Jovanović S, Vukojević J, Marin PD. Chemical composition and antifungal activity of Ssalvia sclarea (lamiaceae) essential oil. Arch Biol Sci. 2008;60(2):233-237. DOI: 10.2298/ABS0802233D.
• [42] Dambolena JS, Zunino MP, López AG, Rubinstein HR, Zygadlo JA, Mwangi JW, Thoithi GN, Kibwage IO, et al. Essential oils composition of Ocimum basilicum L. and Ocimum gratissimum L. from Kenya and their inhibitory effects on growth and fumonisin production by Fusarium verticillioides. Innov. Food Sci. Emerg Technol. 2010;11:410-414. DOI: 10.1016/j.ifset.2009.08.005.
• [43] Sumalan RM, Alexa E, Poiana MA. Assessment of inhibitory potential of essential oils on natural mycoflora and Fusarium mycotoxins production in wheat. Chem Cent J. 2013;7:32. DOI: 10.1186/1752-153X-7-32.
• [44] Yílar M, Kadíođlu I. Antifungal Activities of some Salvia Species Extracts on Fusarium oxysporum f. sp. radicis- lycopersici (Forl) Mycelium Growth In-vitro. Egypt J Biol Pest Co. 2016;26(1):115-118.
• [45] Stević T, Stanković S, Šavikin K, Gođevac D, Dimkić I, Soković M, Berić T. Chemical composition and inhibitory activity of selected essential oils against fungi isolated from Medicinal plants. LEK SIROV. 2014;349(34):69-80.
• [46] Tomescu A, Sumalan RM, Pop G, Alexa E, Poiana MA, Copolovici DM, Mihai CSS, et al. Chemical composition and protective antifugal activity of Mentha Piperita L. and Salvia Officinalis L. essential oils against Fusarium graminearum Spp. REV CHIM. 2015;66(7):1027-1030.
• [47] Pinto E, Salgueiro LR, Cavaleiro C, Palmeira A, Gonc¸alves MJ. In vitro susceptibility of some species of yeasts and filamentous fungi to essential oils of Salvia officinalis. Ind Crop Prod. 2007;26:135-141. DOI: 10.1016/j.indcrop.2007.02.004.
• [48] Rus CF, Pop G, Alexa E, Şumălan RM, Copolovici DM. Antifungal activity and chemical composition of Salvia officinalis l. Essential oil. Res J Agricult Sci. 2015;47(2):186-192.
• [49] Fraternale D, Giamperi L, Bucchini A, Ricci D, Epifano F, Genovese S, Curini M. Composition and antifungal activity of essential oil of Salvia sclarea from Italy. Chem Nat Comp. 2005;41(5):604-606. DOI: 10.1007/s10600-005-0221-9.
• [50] Sharopov FS, Setzer WN. The Essential Oil of Salvia sclarea L. from Tajikistan. Rec Nat Prod. 2012;6(1):75-79.
• [51] Velluti A, Martín S, Gonzalez P, Ramos AJ, Sanchis V. Initial screening for inhibitory activity of essential oils on growth of Fusarium verticillioides, F. proliferatum and F. graminearum on maize-based agar media. Food Microbiol. 2004;21:649-656. DOI: 10.1016/j.fm.2004.03.009.
• [52] Ćosić J, Vrandečić K, Postić J, Jurković D, Ravlić M. In vitro antifungal activity of essential oils on growth of phytopathogenic fungi. POLJOPRIVREDA. 2010;16(2):25-28.
• [53] Juniawan, Sastrahidayat IR, Djauhari S, Himawan T. Inhibition Test of Clove Leaf Oils to Fusarium oxysporum f.sp. cubense. IOSR-JAVS. 2016;9(4):01-07. DOI: 10.9790/2380-0904010107.
• [54] Koul O, Walia S, Dhaliwal GS. Essential oils as green pesticides: Potential and constraints. Biopestic Int.2008;4:63-84.
• [55] Vieira PRN, Morais SM, Bezerra FHQ, Ferreira PAT, Oliveira IR, Silva MGV. Chemical composition and antifungal activity of essential oils from Ocimum species. Ind Crop Prod. 2014;55:267-271. DOI: 10.1016/j.indcrop.2014.02.032.
• [56] Beg AZ, Iqbal Ahmad I. In vitro fungi toxicity of the essential oil of Syzygium aromaticum. World J Microb Biot. 2002;18:313-315. DOI: 10.1023/A:1015242901360.
• [57] Javed S, Mushtaq S, Khokhar I, Ahmad R, Haider MS. Comparative antimicrobial activity of clove and fennel essential oils against food borne pathogenic fungi and food spoilage bacteria. Afr J Biotechnol. 2012;11(94);16065-16070. DOI: 10.5897/AJB11.3058.
• [58] Faria TJ, Ferreira RS, Yassumoto L, Souza JRP, Ishikawa NK, Barbosa AM. Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi. Braz Arch Biol Technol. 2006;49:867-871.
• [59] Camele I, Altieri L, De Martino L, De Feo V, Mancini E, Rana GL. In vitro control of post-harvest fruit rot fungi by some plant essential oil components. Int J Mol Sci. 2012;13:2290-2300. DOI: 10.3390/ijms13022290.
• [60] Soylu EM, Soylu S, Kurt S. Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans. Mycopathologia. 2006;161:119-128. DOI: 10.1007/s11046-005-0206-z.
• [61] Taweechaisupapong S, Ngaonee P, Patsuk P, Pitiphat W, Khunkitti W.. Antibiofilm activity and post antifungal effect of lemongrass oil on clinical Candida dubliniensis isolate. S Afr J Bot. 2012;78:37-43. DOI: 10.1016/j.sajb.2011.04.003.
• [62] Hashem M, Moharam AM, Zaied AA, Saleh FEM. Efficacy of essential oils in the control of cumin root rot disease caused by Fusarium spp. Crop Prot. 2010;29:1111-1117. DOI: 10.1016/j.cropro.2010.04.020.

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).