Influence of cyanobacterial bloom on freshwater biocoenosis. Use of bioassays for cyanobacterial microcystins toxicity assessment

Piontek, M.; Czyżewska, W.

doi:10.1515/ceer-2017-0004

Artykuł - szczegóły

Tytuł artykułu

Influence of cyanobacterial bloom on freshwater biocoenosis. Use of bioassays for cyanobacterial microcystins toxicity assessment

Autorzy

Piontek M. , Czyżewska W.

Treść / Zawartość

Pełne teksty:

ceer_2017_24_piontek_czyzewska_influence.pdf

Pobierz

Identyfikatory

DOI

10.1515/ceer-2017-0004

Warianty tytułu

Wpływ zakwitów sinic na słodkowodną biocenozę. Zastosowanie biotestów do oceny toksyczności mikrocystyn syntetyzowanych przez sinice

Języki publikacji

Abstrakty

The issues presented in this study concern a very important problem of the occurrence of cyanobacterial blooms in surface water used for water supply purposes. The objective of this study was to analyze the occurrence of cyanotoxic risk in the catchment area of the Obrzyca River (including Sławskie lake which is the beginning of the river), which is a source of drinking water for the inhabitants of Zielona Góra. In order to evaluate toxicity of cyanobacterial bloom it was conducted toxicological testing using aquatic invertebrates (Daphnia magna, Dugesia tigrina) and heterotrophic bacteria (Escherichia coli, Enterococcus faecalis, Pseudomonas fluorescens). Test samples were collected from May to October, 2012. The most toxic was a sample collected from Lake Sławskie on 20th October when cyanobacteria bloom with a predominance of Microcystis aeruginosa occurred and the amount of microcystins was the largest. The methanol extract of the sample was toxic only above a concentration of 6x103 μgxdm-3. The lethal concentration (48-h LC 50) for Daphnia magna was 3.09x103 and for Dugesia tigrina (240-h LC 50) 1.51x103μgxdm-3 of microcystins (MC-LR, MC-YR and C-RR). The same extract stimulated growth of Escherichia coli and Enterococcus faecalis cells.

Zagadnienia przedstawione w niniejszym artykule dotyczą bardzo ważnego problemu występowania zakwitów sinic w wodach powierzchniowych wykorzystywanych do celów wodociągowych. Celem pracy była ocena cyjanotoksycznego ryzyka w zlewni rzeki Obrzyca (w tym jeziora Sławskiego będącego początkiem rzeki), która jest źródłem wody do picia dla mieszkańców Zielonej Góry. W celu oceny toksyczności sinic przeprowadzono badania toksykologiczne przy użyciu bezkręgowców wodnych (Daphnia magna i Dugesia tigrina) oraz bakterii heterotroficznych (Escherichia coli, Enterococcus faecalis, Pseudomonas fluorescens). Próbki do badań pobierano od maja do października w 2012 roku. Ekstrakt metanolowy z zakwitu sinic (dominacja gatunku Microcystis aeruginosa) w J. Sławskim, gdzie ilość mikrocystyn była największa, był toksyczny dopiero po zatężeniu do 6•103 μg•dm-3. Stężenie letalne (48-h LC 50) dla Daphnia magna wyniosło 3,09•103 a dla Dugesia tigrina (240-h LC 50) było równe 1,51•103 μg•dm-3 mikrocystyn (MC-LR, MC-YR i MC-RR). Ten sam ekstrakt stymulował wzrost komórek Escherichia coli i Enterococcus faecalis.

Słowa kluczowe

cyanobacteria cyanotoxins toxicity bioassays

sinice toksyczność cyjanotoksyny biotest

Wydawca

Oficyna Wydawnicza Uniwersytetu Zielonogórskiego

Czasopismo

Civil and Environmental Engineering Reports

Rocznik

2017

Tom

No. 24(1)

Strony

49--70

Opis fizyczny

Bibliogr. 52 poz., rys., tab., wykr.

Twórcy

autor

Piontek M.

m.piontek@iis.uz.zgora.pl

Univeristy of Zielona Góra, Zielona Góra, Poland

autor

Czyżewska W.

Water and Wastewater Plant in Zielona Gora, Poland Sp. z o.o., Poland

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Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017)

Typ dokumentu

Bibliografia

Identyfikator YADDA

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