Gradient elution LC-MS determination of dasatinib in rat plasma and its pharmacokinetic study

• Autorzy: Wen C , Zhang Q. , He Y. , Deng M , Wang X. , Ma J.
• Języki publikacji: EN

Abstrakty

EN

A sensitive and simple liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for determination of dasatinib in rat plasma using one-step protein precipitation was developed. After addition of carbamazepine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with methanol-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 488.2 for dasatinib and m/z 338.7 for the IS. Calibration plots were linear over the range of 10–1000 ng mL−1 for dasatinib in rat plasma. Lower limit of quantification (LLOQ) for dasatinib was 10 ng mL−1. Mean recovery of dasatinib from plasma was in the range 82.2%–93.6%. Relative standard deviation (RSD) of intra-day and inter-day precision were both less than 8%. This developed method is successfully used in pharmacokinetic study of dasatinib in rats.

Słowa kluczowe

EN

LC-ESI-MS; rat plasma; gradient elution; dasatinib

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 1
• Strony: 81--91
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Wen C

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Zhang Q.

• Shanghai Institute of Pharmaceutical Industry Shanghai 200437 China

autor

He Y.

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Deng M

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Wang X.

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Ma J.

• Wenzhou Medical College Function Experiment Teaching Center Wenzhou 325035 China

Bibliografia

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