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Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A simple, rapid, and sensitive high-performance liquid chromatography method was developed and validated for the simultaneous determination and quantification of fusidic acid and steroids (prednisone, betamethasone valerate, hydrocortisone acetate, and dexamethasone sodium) from bulk drugs and human plasma. A RP-HPLC, operated at ambient temperature, was equipped with a UV detector for monitoring the effluents at 235 nm. The mobile phase consisted of methanol, acetonitrile, and 0.05 M phosphoric acid (10:60:30, v/v/v), and separation was achieved on a Medeterrane, C18 (5 μm, 12.5 × 0.46 mm) column at a flow rate of 1.7 mL min -1. Calibration curves were linear over concentration range 0.625–10 μg mL -1 with correlation coefficient (r2) greater than 0.9999. The coefficient of variation (CV) and relative error (RE) for intra- and interassay were <2% and <1%, respectively. Interference of other already administered common medicaments, such as aspirin, paracetamol, caffeine, nicotine, and other plasma components, were not found.
Słowa kluczowe
EN
Rocznik
Strony
57--66
Opis fizyczny
Bibliogr. 15 poz., rys., tab.
Twórcy
autor
  • United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE
autor
  • University of Karachi Department of Chemistry Karachi 75270 Pakistan
autor
  • University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan
autor
  • University of Karachi Department of Chemistry Karachi 75270 Pakistan
autor
  • United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE
Bibliografia
  • [1] J.D. Wilkinson. Br J Dermatol., 139, 37 (1998)
  • [2] I. Elsaku, Clin. Chem., 33, 1412 (1987)
  • [3] S.C. Siegel. Clin Rev Allerg., 1, 123–146 (1983)
  • [4] P.L. Toutain. J Chromatogr., 309, 385 (1984)
  • [5] D. S. C. Hui, and J. J. Y. Sung, Chest, 124, 12 (2003)
  • [6] D. S. C. Hui, and J. J. Y. Sung, Chest, 126, 670 (2004)
  • [7] S. T. Lai, Eur. J. Clin. Microbiol. Infect. Dis., 24, 583 (2005)
  • [8] J.Q. Rose, and W.J. Juscko, J. Chromatogr., 162, 273 (1979)
  • [9] M.C. Petersen, R.L. Nation, and J.J. Ashley, J Chromatogr., 183, 131 (1980)
  • [10] I.T. Agabeyoglu, J.G. Wagner, and D.R. Kay, Res. Commun. Chem. Pathol. Pharmacol., 28, 163 (1980)
  • [11] L. Oat, Falk, O. Lantto, and O. Bjorkhem. Scand J. Clin. Lab. Invest., 42, 181 (1982)
  • [12] J. Korpi, D.P. Wittmer, B.J. Sandmann, and W.G. Haney. Jr. J. Pharm. Sci., 65, 1087 (1976)
  • [13] M.D. Smith, J. Chromatogr., 164, 129 (1979)
  • [14] M.S. Arayne, N. Sultana, and M. Nawaz, J. Anal. Chem., 63, 881–887 (2008)
  • [15] The European Agency for the Evaluation of Medical Products. ICH TopicQ2B Note for Guidance on Validation of Analytical Procedures: Methodology GPMP/ICH/281/95, 1996.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-fbac823c-5493-4c4a-be08-8e4c621183ac
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