Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

• Autorzy: Nawaz M. , Arayne M. S. , Sultana N. , Haider A. , Hisaindee S.
• Języki publikacji: EN

Abstrakty

EN

A simple, rapid, and sensitive high-performance liquid chromatography method was developed and validated for the simultaneous determination and quantification of fusidic acid and steroids (prednisone, betamethasone valerate, hydrocortisone acetate, and dexamethasone sodium) from bulk drugs and human plasma. A RP-HPLC, operated at ambient temperature, was equipped with a UV detector for monitoring the effluents at 235 nm. The mobile phase consisted of methanol, acetonitrile, and 0.05 M phosphoric acid (10:60:30, v/v/v), and separation was achieved on a Medeterrane, C18 (5 μm, 12.5 × 0.46 mm) column at a flow rate of 1.7 mL min ^-1. Calibration curves were linear over concentration range 0.625–10 μg mL ^-1 with correlation coefficient (r2) greater than 0.9999. The coefficient of variation (CV) and relative error (RE) for intra- and interassay were <2% and <1%, respectively. Interference of other already administered common medicaments, such as aspirin, paracetamol, caffeine, nicotine, and other plasma components, were not found.

Słowa kluczowe

EN

fusidic acid; steroids; HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 1
• Strony: 57--66
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Nawaz M.

• United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE

autor

Arayne M. S.

• University of Karachi Department of Chemistry Karachi 75270 Pakistan

autor

Sultana N.

• University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan

autor

Haider A.

• University of Karachi Department of Chemistry Karachi 75270 Pakistan

autor

Hisaindee S.

• United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE

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