Development and validation of an HPTLC method for analysis of zerumbone, the anticancer marker from Zingiber zerumbet

• Autorzy: Rout K. K , Mishra S. K , Sherma J
• Języki publikacji: EN

Abstrakty

EN

A simple, accurate, and rapid high-performance thin-layer chromatographic (HPTLC) method for quantification of the anticancer marker zerumbone in different parts of Zingiber zerumbet Smith has been established and validated. Chromatography was performed on aluminium foil-backed silica gel 60 F 254 HPTLC plates with the binary mobile phase ethyl acetate-hexane 1.5:8.5. Ultraviolet detection was performed densitometrically at the maximum absorbance wavelength, 250 nm. Regression analysis data for the calibration plot showed there was a linear relationship between response and concentration in the range 60–260 ng with a regression coefficient of 0.9997. The limits of detection and quantification were 20 and 60 ng, respectively. The instrumental precision and repeatability of the method were found to be 0.80 and 1.08%, respectively. Recovery ranging from 97.85 to 100.12% indicated the excellent accuracy of the method. The developed HPTLC method was successfully used for analysis of the marker in different parts of Z. zerumbet , and the maximum zerumbone content (1.81%) was found in the rhizome.

Słowa kluczowe

EN

high-performance thin-layer chromatography; HPTLC; densitometry; anticancer marker; zerumbone; Zingiber zerumbet

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2009
• Tom: Vol. 21, no. 3
• Strony: 443--452
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Rout K. K

• Utkal University Pharmacognosy and Phytochemistry Division, University Department of Pharmaceutical Sciences Bhubaneswar 751004 Orissa India

autor

Mishra S. K

• Utkal University Pharmacognosy and Phytochemistry Division, University Department of Pharmaceutical Sciences Bhubaneswar 751004 Orissa India

autor

Sherma J

• Lafayette College Department of Chemistry Easton PA 18042-1782 USA

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