Exposure to High-molecular-weight Polyvinyl Chloride Alters Bacterial Diversity in the Gut Microbiota of the Wistar Rat

• Autorzy: Pekmezekmek Ayper Boga , Emre Mustafa , Tunc Erdal , Kalay Betül , Kocahan Sayad , Emre Nesrin , Emre Toygar

Identyfikatory

DOI

10.54740/ros.2024.038

• Języki publikacji: EN

Abstrakty

EN

The physical and chemical characteristics of microplastics make it easier for contaminants to adhere to the surface of the particles, acting as a vehicle for toxins to reach organisms after ingestion. The "most microbiome" comprises all the microorganisms present in our bodies as a whole because it has a big surface area and provides nutrientrich components for the digestive system's germs. In this investigation, metagenome analysis was used to determine the impact of long-term administration of High-Molecular Weight-Polyvinyl Chloride microplastics to young Wistar rats on the gut microbiota. Forty adult rats in total were employed, with 15 first-group and 15 second-group experimental groups and 10 controls. Pellets made specifically for feeding rats are produced. Following the procedure, the rats were anaesthetised with ketamine and xylasine before being dissected. Due to the small number of samples, alpha diversity in the gut metagenome study did not demonstrate statistically significant variations, but it did illustrate differences in bacterial diversity and density. In particular, it has been discovered that bacterial diversity is higher in experimental groups. According to the control groups, in the assay groups, the intestinal microbiome, dominated by Escherichia coli, Shigella, and Lactobacillus, was assessed as an increase in metabolic pathways related to microplastic exposure and pathogenicity in general. The findings demonstrate the necessity for extreme caution in the manufacture and use of plastics that pose a risk to the welfare of living things.

Słowa kluczowe

EN

HMW-PVC; intestine; krona; metagenome; microbiota

• Wydawca: Politechnika Koszalińska
• Czasopismo: Rocznik Ochrona Środowiska
• Rocznik: 2024
• Tom: Tom 26
• Strony: 393--402
• Opis fizyczny: Bibliogr. 50 poz., rys., tab.

Twórcy

autor

Pekmezekmek Ayper Boga

• Department of Physiology, School of Medicine, Cukurova University, Adana, Türkiye

• https://orcid.org/+0000-0002-3028-605X

autor

Emre Mustafa

• Department of Biophysics, School of Medicine, Cukurova University, Adana, Türkiye

• https://orcid.org/0000-0001-9897-6674

autor

Tunc Erdal

• Department of Medical Biology and Genetics, School of Medicine, Artuklu University, Mardin, Türkiye

• https://orcid.org/0000-0003-4964-1004

autor

Kalay Betül

• Department of Pharmacology, School of Medicine, Cukurova University, Adana, Türkiye

• https://orcid.org/0009-0009-4085-3423

autor

Kocahan Sayad

• Department of Physiology, Gülhane Faculty of Medicine, University of Health Sciences, Ankara, Türkiye

• https://orcid.org/0000-0002-3161-1280

autor

Emre Nesrin

• Department of Mathematics and Science Education, Faculty of Education, Akdeniz University, Antalya, Türkiye

• https://orcid.org/0000-0001-9047-1823

autor

Emre Toygar

• Department of Industry, Faculty of Engineering, Boğaziçi University, İstanbul, Türkiye

• https://orcid.org/0000-0002-2577-5879

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