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Optimized ultra-high-performance liquid chromatography tandem mass spectrometry method for detecting compositional changes in Eucommia ulmoides and Achyranthes bidentata paired decoctions in vitro and in vivo

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Rationale: The bark of Eucommia ulmoides and the roots of Achyranthes bidentata are commonly used in traditional Chinese medicine, and their pairing appears in many traditional Chinese medicine formulas as a recognized compatible unit. However, the changes and interactions of the main components of these two formulas when paired remain unclear, and there is currently no standard or method for their quality control and assessment of pharmacological effects. Methods: An optimized ultra-high-performance liquid chromatography triple-quadrupole mass spectrometry (UHPLC-MS/MS) method was established for the simultaneous identification of 10 components in E. ulmoides and A. bidentata using in vitro and in vivo models. Tributyltin methacrylate was the internal standard solution, and the blood samples were treated by an organic solvent precipitation method. Gradient elution was conducted on a C₁₈ column at 25 °C with 0.1% formic acid water:acetonitrile as the mobile phase at a flow rate of 0.5 mL min⁻¹. Dynamic multiple response monitoring was performed in negative-ion mode using an Agilent Jet Stream electrospray ionization ion source. Results: In negative-ion detection mode, eucommiol exhibited a good response, and the isomers ginsenoside Ro and achyranthoside C could also be well separated. The developed method accurately detected the five components with a low blood content. Compared to controls, the levels of ginsenoside Ro, chikusetsusaponin Ⅳa, and achyranthoside C increased; the contents of geniposidic acid and pinoresinol diglucoside were unchanged; and the levels of eucommiol, geniposide, β-ecdysterone, genipin, and achyranthoside D decreased in vitro. In vivo, the contents of geniposidic acid, geniposide, pinoresinol diglucoside, and β-ecdysterone were reduced; the contents of eucommiol and ginsenoside Ro were unchanged; and those of achyranthoside D, chikusetsusaponin Ⅳa, and achyranthoside C increased compared to the corresponding levels in the internal control. Conclusions: A method for the quality control of the E. ulmoides-A. bidentata drug pair was established for the first time and the main components in 10 drug pairs could be determined simultaneously in vitro and in vivo. These findings show that the E. ulmoides and A. bidentata drug pair cause a compositional change, providing new ideas for the development of this combination to improve clinical efficacy.
Rocznik
Strony
31--44
Opis fizyczny
Bibliogr. 29 poz., tab., wykr.
Twórcy
autor
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
  • Jiangxi University of Chinese Medicine, Nanchang, China
autor
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
autor
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
  • Jiangxi University of Chinese Medicine, Nanchang, China
autor
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
  • Jiangxi University of Chinese Medicine, Nanchang, China
autor
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
  • Jiangxi University of Chinese Medicine, Nanchang, China
autor
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
  • Jiangxi University of Chinese Medicine, Nanchang, China
autor
  • Department of Pharmacy, Third Affiliated Hospital of Naval Medical University, Shanghai, China
  • Jiangxi University of Chinese Medicine, Nanchang, China
Bibliografia
  • 1. Duan, J.; Su, S.; Tang, Y.; Fan, X. Modern understanding of compatibility combination of traditional Chinese medicine. J. Nanjing Univ. Tradit. Chin. Med. 2009, 25(05), 330–3. https://kns.cnki.net/kcms/detail/detail.aspx?FileName5NJZY200905002&DbName5CJFQ2009.
  • 2. Xu, Q. The Couplet Medicines of Traditional Chinese Medicine Encyclopedia, Vol. Aug; China Press of Traditional Chinese Medicine Co: Beijing, 1981.
  • 3. Huang, L.; Lyu, Q.; Zheng, W.; Yang, Q.; Cao, G. Traditional application and modern pharmacological research of Eucommia ulmoides oliv. Chin. Med. 2021, 16(1), 73. https://doi.org/10.1186/s13020-021-00482-7.
  • 4. Chen, Z.; Wu, G.; Zheng, R. A systematic pharmacology and in vitro study to identify the role of the active compounds of Achyranthes bidentata in the treatment of osteoarthritis. Med. Sci. Monit. 2020, 26. https://doi.org/10.12659/MSM.925545.
  • 5. Wu, D.; Yu, D.; Zhang, Y.; Dong, J.; Li, D.; Wang, D. Metabolite profiles, bioactivity, and HPLC fingerprint of different varieties of Eucommia ulmoides oliv.: towards the utilization of medicinal and commercial Chinese endemic tree. Molecules 2018, 23(8), 1898. https://doi.org/10.3390/molecules23081898.
  • 6. Fu, J.; Wu, H.; Wu, H.; Deng, R.; Sun, M. Deciphering the metabolic profile and pharmacological mechanisms of Achyranthes bidentata blume saponins using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry coupled with network pharmacology-based investigation. J. Ethnopharmacol. 2021, 274, 114067. https://doi.org/10.1016/j.jep.2021.114067.
  • 7. Hu, F.; An, J.; Li, W.; Zhang, Z.; Chen, W.; Wang, C.; Wang, Z. UPLC-MS/MS determination and gender-related pharmacokinetic study of five active ingredients in rat plasma after oral administration of Eucommia cortex extract. J. Ethnopharmacol. 2015, 169, 145–55. https://doi.org/10.1016/j.jep.2015.04.007.
  • 8. Tao, Y.; Du, Y.; Li, W.; Cai, B. Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: application to a comparative pharmacokinetic study of aqueous extracts of raw and salt-processed Achyranthes bidentata. J. Pharm. Biomed. Anal. 2018, 151, 164–9. https://doi.org/10.1016/j.jpba.2017.12.024.
  • 9. Xu, X.-X.; Zhang, X.-H.; Diao, Y.; Huang, Y.-X. Achyranthes bidentate saponins protect rat articular chondrocytes against interleukin-1β-induced inflammation and apoptosis in vitro. Kaohsiung J. Med. Sci. 2017, 33(2), 62–8. https://doi.org/10.1016/j.kjms.2016.11.004.
  • 10. Wu, M.; Zhao, S.; Ren, L.; Wang, R.; Bai, X.; Han, H.; Li, B.; Chen, H. Research on relationship between tissue quantitative distribution of 3H-Achyranthes bidentata ecdysterone and channel-tropism of herbal drugs in mice. Zhongguo Zhong Yao Za Zhi 2011, 36(21), 3018–22, PMID: 22308694.
  • 11. Li, X.; Zhu, W.; Yang, L.; Fei, D.; Fan, J.; Du, L.; Liu, Y. Evaluation of the sedative and hypnotic effects of eucommiol in Eucommia. Nat. Product. Res. 2013, 27(18), 1657–9. https://doi.org/10.1080/14786419.2012.746343.
  • 12. Li, Y.; Kamo, S.; Metori, K.; Koike, K.; Che, Q.; Takahashi, S. The promoting effect of eucommiol from eucommiae cortex on collagen synthesis. Biol. Pharm. Bull. 2000, 23(1), 54–9. https://doi.org/10.1248/bpb.23.54.
  • 13. Hsu, H.-Y.; Yang, J.-J.; Lin, S.-Y.; Lin, C.-C. Comparisons of geniposidic acid and geniposide on antitumor and radioprotection after sublethal irradiation. Cancer Lett. 1997, 113(1–2), 31–7. https://doi.org/10.1016/S0304-3835(96)04572-7.
  • 14. Habtemariam, S.; Lentini, G. Plant-derived anticancer agents: lessons from the pharmacology of geniposide and its aglycone, genipin. Biomedicines 2018, 6(2), 39. https://doi.org/10.3390/biomedicines6020039.
  • 15. He, G.; Guo, W.; Lou, Z.; Zhang, H. Achyranthes bidentata saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK MAPK signaling pathway. Cell Biochem Biophys 2014, 70(1), 467–73. https://doi.org/10.1007/s12013-014-9942-3.
  • 16. Li, S. Effects of Geniposide, Achyranthes Bidentata Saponions and Their Combination on the Expression of Filamentous Actin in Fibroblast-like Synovicytes of Adjuvant Arthritis Rats; Anhui University of Traditional Chinese Medicine: Anhui AH, 2017. https://kns.cnki.net/kcms/detail/detail.aspx?FileName51017199133.nh&DbName5CMFD2018.
  • 17. Jiang, L.; Li, W.; Zhuang, T.; Yu, J.; Sun, S.; Ju, Z.; Wang, Z.; Ding, L.; Yang, L. Ginsenoside Ro ameliorates high-fat diet–induced obesity and insulin resistance in mice via activation of the G protein–coupled bile acid receptor 5 pathway. J. Pharmacol. Exp. Ther. 2021, 377(3), 441–51. https://doi.org/10.1124/jpet.120.000435.
  • 18. Yuan, C.; Liu, C.; Wang, T.; He, Y.; Zhou, Z.; Dun, Y.; Zhao, H.; Ren, D.; Wang, J.; Zhang, C.; Yuan, D. Chikusetsu saponin IVa ameliorates high fat diet-induced inflammation in adipose tissue of mice through inhibition of NLRP3 inflammasome activation and NF-KB signaling. Oncotarget 2017, 8(19), 31023–40. https://doi.org/10.18632/oncotarget.16052.
  • 19. Wang, S.; Zeng, M.; Li, B.; Kan, Y.; Zhang, B.; Zheng, X.; Feng, W. Raw and salt-processed Achyranthes bidentata attenuate LPS-induced acute kidney injury by inhibiting ROS and apoptosis via an estrogen-like pathway. Biomed. Pharmacother. 2020, 129, 110403. https://doi.org/10.1016/j.biopha.2020.110403.
  • 20. Kim, B.-C.; Kim, H.-G.; Lee, S.-A.; Lim, S.; Park, E.-H.; Kim, S.-J.; Lim, C.-J. Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway. Biochem. Pharmacol. 2005, 70(9), 1398–407. https://doi.org/10.1016/j.bcp.2005.07.025.
  • 21. Lin, F.; Wang, Y-H.; Wan, L.; Yang, R-P. Study on the quality control of eucommiae cortex by multi-components quantitation by one marker method and fingerprint. Chin. J. Exp. Traditional Med. Formulae 2012, 18(13), 78–82. https://doi.org/10.13422/j.cnki.syfjx.2012.13.027.
  • 22. Zhang, M.; Zhao, H.; Zhou, S. Content determination of β-ecdysterone and oleanolic acid in Achyranthes bidentata blume by HPLC and their fingerprints. Shandong Sci. 2015, 28(05), 1–6. https://doi.org/10.3976/j.issn.1002-4026.2015.05.001.
  • 23. Zhang, C.; Liang, S.; Zhang, G. Determination of ecdysterone in Achyranthes bidentata from different locations. Chin. J. Pharm. 2001, 10, 52–3. https://kns.cnki.net/kcms/detail/detail.aspx?FileName5ZGYX200110018&DbName5CJFQ2001.
  • 24. Xie, G.; Jiang, N.; Wang, S.; Qi, R.; Wang, L.; Zhao, P.; Liang, L.; Yu, B. Eucommia ulmoides oliv. Bark aqueous extract inhibits osteoarthritis in a rat model of osteoarthritis. J. Ethnopharmacol. 2015, 162, 148–54. https://doi.org/10.1016/j.jep.2014.12.061.
  • 25. Wang, Y.; Zhang, M.; Zha, L.; Guo, Y. Research on the extraction process of polysaccharides in cyathulae radix and Achyranthes bidentatae radix. J. Changchun Normal Univ. 2016, 35(10), 77–81. https://kns.cnki.net/kcms/detail/detail.aspx?FileName5CCSS201610018&DbName5CJFQ2016.
  • 26. Zhang, X.-H.; Xu, X.-X.; Xu, T. Ginsenoside Ro suppresses interleukin-1β-induced apoptosis and inflammation in rat chondrocytes by inhibiting NF-KB. Chin. J. Nat. Medicines 2015, 13(4), 283–9. https://doi.org/10.1016/S1875-5364(15)30015-7.
  • 27. Zhang, X.; Wang, L.; Song, F.; Liu, Z.; Liu, S. Study on the variation of chemical constituents during combination of ginseng with Trogopteroum feces and semen Raphani by high performance liquid chromatography mass spectrometry. Chin. J. Anal. Chem. 2007, 35(4), 559–63. https://doi.org/10.3321/j.issn:0253-3820.2007.04.021.
  • 28. Tang, Y.; Mo, Y.; Xin, D.; Zeng, L.; Yue, Z.; Xu, C. β-Ecdysterone alleviates osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/MTOR signal pathway. Am. J. Transl Res. 2020, 12(11), 7174–86, PMCID: PMC7724317.
  • 29. Chen, M.-Y.; Shao, L.; Zhang, W.; Wang, C.-Z.; Zhou, H.-H.; Huang, W.-H.; Yuan, C.-S. Metabolic analysis of panax notoginseng saponins with gut microbiota-mediated biotransformation by HPLC-DAD-Q-TOF-MS/MS. J. Pharm. Biomed. Anal. 2018, 150, 199–207. https://doi.org/10.1016/j.jpba.2017.12.011.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-f93a83c7-5c89-4b09-a97e-908a3a385943
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