Shape memory process in resorbable polymers: effect on surface properties and cell adhesion

• Autorzy: Costa A. M. , Ferreira A. S. , Posadowska U. , Krok M. , Smola A. , Dobrzyński P. , Pamuła E.
• Języki publikacji: EN

Abstrakty

EN

The objectives of this study were to confirm the shape memory behavior of two new bioresorbable terpolymers (L-lactide, glycolide, and trimethylene carbonate: L-PLGTMC and B-PLGTMC), to follow the influence of the shape memory process on their surface properties and to test their cytocompatibility using osteoblast-like cells. For this purpose, foils of both terpolymers were prepared. The terpolymers' ability to recover up to 92-93% of the memorized shape within 10 seconds was obtained. The influence of shape memory process on the surface properties was assessed by water contact angle (WCA) measurement and atomic force microscopy (AFM) and the results suggested that both terpolymers preserved the hydrophilicity after recovery and also that B-PLGTMC polymer was rougher than L-PLGTMC (about 9 folds more). The AFM pictures showed the presence of spherical shape hills on the B-PLGTMC foil surface which after the stretching procedure became oriented toward the direction of the applied load. The terpolymers were seeded on both sides (Top and Bottom faces) with human MG63 osteoblast-like cells. Cell viability was assessed after 1, 3 and 7 days, using MTT assay. Results revealed an increasing number of metabolically active cells with the incubation time, suggesting, together with nitric oxide (NO) level determination, the cytocompatibility of both terpolymers. Cell spreading and morphology were investigated by H&E staining and obtained results corresponded well with ones of MTT and NO.

Słowa kluczowe

EN

shape memory; PLGA; tissue regeneration; foil

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2012
• Tom: Vol. 15, no. 114
• Strony: 8--11
• Opis fizyczny: Bibliogr. 19 poz., rys., tab., wykr.

Twórcy

autor

Costa A. M.

• Bioengineering, Faculty of Engineering, UP - University of Porto, Reitoria da U. Porto Praca Gomes Teixeira, Porto 4099-002, Portugal
• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. A. Mickiewicza 30, 30-059 Krakow, Poland

autor

Ferreira A. S.

• Bioengineering, Faculty of Engineering, UP - University of Porto, Reitoria da U. Porto Praca Gomes Teixeira, Porto 4099-002, Portugal
• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. A. Mickiewicza 30, 30-059 Krakow, Poland

autor

Posadowska U.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. A. Mickiewicza 30, 30-059 Krakow, Poland

autor

Krok M.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. A. Mickiewicza 30, 30-059 Krakow, Poland

autor

Smola A.

• Centre of Polymer and Carbon Materials, Polish Academy of Sciences, ul. M. CuRiE Skłodowskiej 34, 41-800 Zabrze, Poland

autor

Dobrzyński P.

• Centre of Polymer and Carbon Materials, Polish Academy of Sciences, ul. M. CuRiE Skłodowskiej 34, 41-800 Zabrze, Poland
• Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University in Częstochowa, al. Armii Krajowej 13/15, 42-200 Częstochowa, Poland

autor

Pamuła E.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. A. Mickiewicza 30, 30-059 Krakow, Poland

Bibliografia

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Uwagi

EN

This study was supported from Polish National Science Center (2011/01/B/ST5/06296).