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Metagenomic Analysis Reveals Microbial Communities in Lake Qarun – Egypt

Treść / Zawartość
Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Characterization of water quality in Lake Qarun indicated that the water is very poor for irrigation and aquatic life. At the same time, the bacterial community was represented mainly by five bacterial phyla with different proportions: Firmicutes (53%), Proteobacteria (33%), Bacteroidetes (7%), Actinobacteria (5%) and Thermi (1%). Furthermore, metagenomes prediction of bacterial communities using PICRUSt indicated important functional gene families associated with metabolism, environmental information, genetic information processing, and cellular processes. It is worth noting that Benzoate degradation had the highest average relative abundance, followed by aminobenzoate degradation among 18 individual KEGG pathways from xenobiotics biodegradation and metabolism which showed higher relative abundance. The obtained data indicate that a different source of pollution in Qarun Lakes has an impact on the bacterial community’s structure, as well as the biota and is expected to cause health problems.
Rocznik
Strony
70--76
Opis fizyczny
Bibliogr. 27 poz., rys., tab.
Twórcy
  • Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
  • Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
  • Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
Bibliografia
  • 1. Mageed A.A. 1998. Distribution and Salinity Ranges of Zooplankton Organisms at El-Fayoum Depression (El-Fayoum-Egypt). Egyptian Journal of Aquatic Biology and Fisheries, 2(2), 51–71.
  • 2. Wahed S.M.A., Mohamed E.A., Wolkersdorfer C., El-Sayed M.I., M’nif A., Sillanpää M. 2015. Assessment of Water Quality in Surface Waters of the Fayoum Watershed, Egypt. Environmental Earth Sciences, 74(2), 1765–1783.
  • 3. Al-Afify A.D.G., Tahoun U.M., Abdo M.H. 2019. Water Quality Index and Microbial Assessment of Lake Qarun, El-Batts and El-Wadi Drains, Fayoum Province, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 23(1), 341–357.
  • 4. Ali F.K., El-Shafai S.A., Samhan F.A.,. Khalil W.K.B. 2010. Effect of Water Pollution on Expression of Immune Response Genes of Solea Aegyptiaca in Lake Qarun. African Journal of Biotechnology, 7(10), 1418–1425.
  • 5. Araya R., Katsuji T., Tatsuya T., Nobuyasu Y., Masao N. 2003. Bacterial Activity and Community Composition in Stream Water and Biofilm from an Urban River Determined by Fluorescent in Situ Hybridization and DGGE Analysis. FEMS Microbiology Ecology, 43(1), 111–119.
  • 6. Cabello-Yeves P.J., Zemskaya T.I., Zakharenko A.S., Sakirko M.V., Ivanov V.G., Ghai R., Rodriguez- Valera F. 2020. Microbiome of the Deep Lake Baikal, a Unique Oxic Bathypelagic Habitat. Limnology and Oceanography, 65(7), 1471–1488.
  • 7. Caporaso J.G., Kuczynski J., Stombaugh J., Bittinger K., Bushman F.D., Costello E.K., Fierer N., et al. 2010. QIIME Allows Analysis of High-Throughput Community Sequencing Data. Nature Methods, 7(5), 335–336.
  • 8. Dang V.T., Speck P., Benkendorff K. 2012. Influence of Elevated Temperatures on the Immune Response of Abalone, Haliotis Rubra. Fish & Shellfish Immunology, 32(5), 732–740.
  • 9. El-Noby G.A., Hassanin M., El-Hady M., Aboshabana S. 2021. Streptococcus: A Review Article on an Emerging Pathogen of Farmed Fishes. Egyptian Journal of Aquatic Biology and Fisheries, 25(1), 123–139.
  • 10. El-Shabrawy G., Gohar M., Germoush M., Anufriieva E., Shadrin N. 2014. Long-Term Changes in the Saline Lake Qarun (Egypt). Acta Geologica Sinica, 88, 180–182.
  • 11. Fantini E., Gianese G., Giuliano G., Fiore A. 2015. Bacterial Metabarcoding by 16S RRNA Gene Ion Torrent Amplicon Sequencing. Methods in Molecular Biology, 1231, 77–90.
  • 12. Goher M.E., El-Rouby W.M.A., El-Dek S.I., El-Sayed S.M., Noaemy S.G. 2018. Water Quality Assessment of Qarun Lake and Heavy Metals Decontamination from Its Drains Using Nanocomposites. Materials Science and Engineering, 464(1).
  • 13. Gupta G., El-Hamid Z.A. 2003. Water Quality of Lake Qarun, Egypt.International Journal of Environmental Studies, 60(6), 651–657.
  • 14. Lubna I., Ramzy E.M. 2013. Water Quality and Its Impact on Tilapia Zilli (Case Study) Qarun Lake- Egypt. International Water Technology Journal, 3(2),
  • 15. Kirs M., Harwood V.J., Fidler A.E., Gillespie P.A., Fyfe W.R., Blackwood A.D., Cornelisen C.D. 2011. Source Tracking Faecal Contamination in an Urbanised and a Rural Waterway in the Nelson-Tasman Region, New Zealand. New Zealand Journal of Marine and Freshwater Research, 45(1), 43–58.
  • 16. Köchling T., Sanz J.L., Galdino L., Florencio L., Kato M.T. 2017. Impact of Pollution on the Microbial Diversity of a Tropical River in an Urbanized Region of Northeastern Brazil. International Microbiology, 20(1), 11–24.
  • 17. Mansour S.A., Sidky M.M. 2003. Ecotoxicological Studies. 6. The First Comparative Study between Lake Qarun and Wadi El-Rayan Wetland (Egypt), with Respect to Contamination of Their Major Components. Food Chemistry. Elsevier, 82(2), 181–189.
  • 18. McDonald D., Price M.N., Goodrich J., Nawrocki E.P., Desantis T.Z., Probst A., Andersen G.L., Knight R., Hugenholtz P. 2012. An Improved Greengenes Taxonomy with Explicit Ranks for Ecological and Evolutionary Analyses of Bacteria and Archaea. ISME Journal, 6(3), 610–618.
  • 19. Authman M.M., Abbas H. 2007. Accumulation and Distribution of Copper and Zinc in Both Water and Some Vital Tissues of Two Fish Species (Tilapia Zillii and Mugil Cephalus) of Lake Qarun, Fayoum Province, Egypt. Pakistan Journal of Biological Sciences: PJBS, 10(13), 2106–2122.
  • 20. Samson M.K., Maina S., Aswathy S., Schilling M., Kapur V., Gwakisa P. 2016. High-Throughput Sequencing of 16S RRNA Gene Reveals Substantial Bacterial Diversity on the Municipal Dumpsite. BMC Microbiology, 16(1), 1–12.
  • 21. Dhiraj P., Kumbhare S.V., Mhatre S.S., Chowdhury S.P., Shetty S.A., Marathe N.P., Bhute S., Shouche Y.S. 2016. Exploration of Microbial Diversity and Community Structure of Lonar Lake:The Only Hypersaline Meteorite Crater Lake within Basalt Rock. Frontiers in Microbiology. Frontiers, 1553.
  • 22. Schuster S.C. 2008. Next-Generation Sequencing Transforms Today’s Biology. Nature Methods, 5(1), 16–18.
  • 23. Shaaban M.T., Hanafi A.A.M., Ibrahim H.A.H. 2016. Distribution of Bacteria in Lake Qarun, AL Fayoum, Egypt (2014–2015) in Relation to Its Physical and Hydrochemical Characterization. Journal of Bioscience and Applied Research, 2(9), 601–615.
  • 24. Neethu S., Tang H., Doak T.G., Ye Y. 2011. Comparing Bacterial Communities Inferred from 16S RRNA Gene Sequencing and Shotgun Metagenomics.proc. Pacific Symposium on Biocomputing, 165–176.
  • 25. Thermo Fisher, Ion PGM Hi‑Q View OT2 Kit User Guide (Pub. No. MAN0014579 Rev. C.0)
  • 26. François T., Hehemann J.H., Rebuffet E., Czjzek M., Michel G. 2011. Environmental and Gut Bacteroidetes. The Food Connection. Frontiers in Microbiology, 2, 93.
  • 27. Qiong W., Garrity G.M., Tiedje J.M., Cole J.R. 2007. Naïve Bayesian Classifier for Rapid Assignment of RRNA Sequences into the New Bacterial Taxonomy. Applied and Environmental Microbiology, 73(16), 5261–5267.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-f6c7a964-b7e8-4a15-8f72-e214da9972b5
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