Use of Escherichia Coli K-12 Strain With Gfp Reporter Gene for Screening of Cefuroxime and Metronidazole Residues in Surface Water

• Autorzy: Matejczyk M. , Rosochacki S. J. , Jabłonowski K.

Identyfikatory

DOI

10.12911/22998993/2933

• Języki publikacji: EN

Abstrakty

EN

Antibiotic residues are constantly detected in environmental waters at relatively low concentrations. They can modulate the biological stability of ecosystems after entering the environment, therefore robust and reliable methods for determining their residues in environmental samples are required. In this work, Escherichia coli K-12 GFP-based bacterial biosensors strain was used to detect cyto- and genotoxic activity of cefuroxime and metronidazole at concentration of 500, 1000, 2000, 3000 and 5000 μg/ml. Experimental data indicated that metronidazole, at higher concentrations of 3000 and 5000 μg/ml showed stronger cyto- and genotoxix activity than cefuroxime to bacteria cells. Incubation of bacteria cells with surface water with both drugs modulated gfp gene expression. E. coli K-12 strain with genetic fusion of recA promoter and gfp reporter gene was a good model organism for screening cyto- and genotoxic effect of cefuroxime and metronidazole in applied in this experiment concentrations of these drugs.

Słowa kluczowe

EN

recA; gfp biosensor; cytotoxicity; genotoxicity; cefuroxime; metronidazole

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2015
• Tom: Vol. 16, nr 3
• Strony: 77--82
• Opis fizyczny: Bibliogr. 17 poz., tab., rys.

Twórcy

autor

Matejczyk M.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, Wiejska 45E, 15-351 Bialystok, Poland

autor

Rosochacki S. J.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, Wiejska 45E, 15-351 Bialystok, Poland

autor

Jabłonowski K.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, Wiejska 45E, 15-351 Bialystok, Poland

Bibliografia

• 1. Alhadrami H.A., Paton G.I. 2013. The potential applications of SOS-lux biosensors for rapid screening of mutagenic chemicals. FEMS Microbiol. Lett., 344, 1, 69–76.
• 2. Brunton L., Chabner B., Knollman B. 2010. Goodman and Gilman’s The Pharmacological Basis of Therapeutics; McGraw-Hill Book Co.: New York, NY, USA, 235–249.
• 3. Fatta-Kassinos D., Meric S., Nikolaou A. 2011. Pharmaceutical residues in environmental waters and wastewater: current state of knowledge and future research. Anal Bioanal Chem, 399, 251–275.
• 4. Faure C., Perreira D., Audo I. 2015. Retinal toxicity after intracameral use of a standard doseof cefuroxime during cataract surgery. Doc. Ophthalmol., 130, 57–63.
• 5. Grosa M., Rodríguez-Mozaz S., Barceló D. 2013. Rapid analysis of multiclass antibiotic residues and some of their metabolites in hospital, urban wastewater and river water by ultra-high-performance liquid chromatography coupled to quadrupole-linear ion trap tandem mass spectrometry. J Chromatograph. A, 1292, 173–188.
• 6. Isidori M., Bellotta M., Cangiano M., Parrella A. 2009. Estrogenic activity of pharmaceuticals in the aquatic environment. Environ. Int., 35, 826–829.
• 7. Kessler R.E., Bies M., Chisbolm D.R., Pursiano T.A., Tsai Y.H., Misiek M. 1985. Comparison of a new cephalosporin, BMY 28142, with other broad-spectrum β-lactam antibiotics. Antimicrob. Agents Chemother., 27, 207–216.
• 8. Kim S., Aga D.S. 2007. Potential Ecological and Human Health Impacts of Antibiotics and Antibiotic-Resistant Bacteria from Wastewater Treatment Plants. J. Toxicol. Environ. Health B: Crit. Rev., 10, 559–573.
• 9. Kostrzyńska M., Leung K.T., Lee H., Trevors J.T. 2002. Green fluorescence protein based biosensor for detecting SOS-inducing activity of genotoxic compounds. J Microbiol. Meth., 48, 43–51.
• 10. Liu X., Lee J., Ji K., Takeda S., Choi K. 2012. Potentials and mechanisms of genotoxicity of six pharmaceuticals frequently detected in freshwater environment. Toxicol. Lett., 211, 70–76.
• 11. Matejczyk M. 2010. The Potency of application of microbial biosensors. Advances in Microbiology (In Polish), 4, 297-304.
• 12. Mondragon V.A., Llamas-Perez D.F., Gonzalez- Guzman G.E., Marquez Gonzalez A.R., Padilla- Noriega R., Duran-Avelar M.D., Franco B. 2011. Identification of Enterococcus faecalis bacteria resistant to heavy metals and antibiotics in surface waters of the Mololoa River in Tepic, Nayarit, Mexico. Environ. Monit. Assess., 183, 329–340.
• 13. Okamoto M.P., Nakahiro R.K., Chin A., Bedikian A., Gill M.A. 1994. Cefepime: A new fourth-generation cephalosporin. Am. J. Hosp. Pharm., 51, 463–477.
• 14. Park M., Tsai S.L., Chen W. 2013. Microbial Biosensors: Engineered microorganisms as the sensing machinery. Sensors, 13: 5777-5795.
• 15. Ptitsyn L.R., Horneck G., Komova O., Kozubek S., Krasavin E.A., Bonev M., Rettberg P. 1997. A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells. Appl. Environm. Microbiol., 63, 4377–4384.
• 16. Wagil M., Maszkowska J., Białk-Bielinska A., Caban M., Stepnowski P., Kumirska J. 2015. Determination of metronidazole residues in water, sediment and fish tissue Samales. Chemosphere, 119, 28–34.
• 17. Zaslaver A., Mayo A.E., Rosemberg R., Bashkin P., Sberro H., Tsalyuk M., Surette M.G., Alon U. 2004. Just-in-time transcription program in metabolic pathways. Nat. Genet., 36, 5, 486–491.