Enhancing gamma-decalactone production by protoplast fusion of Yarrowia lipolytica

• Autorzy: Gamil I. , Khatteb A. E.-N. , Shaaban M. , Abd El-Mageed M. , Talkhan F. , Osman F.
• Języki publikacji: EN

Abstrakty

EN

γ-Decalactone is an aroma compound with a pleasant peachy odour, it can be produced by Yarrowia lipolytica, but the yield is commonly poor. Therefore, the present study aimed to use protoplast fusion performed between different superior mutants of Y. lipolytica to improve γ-decalactone production. Data showed that 45 fusants obtained from all three crosses, the cross 1 enhanced the lipase relative production (C/G) up to 106.24% for the fusant C1/9 over the original strain. Furthermore, the highest record of the lipase production was 9.50% based on the lipase relative production (C/G), with 227.82% more than the original strain, which obtained from the fusant No. C2/3 (cross2). Moreover, the highest record of the lipase production was 10 based on the lipase relative production (C/G), with 239.81% more than the wild type strain, which obtained from the fusants C3/2 and C3/14 (cross3). Finally, the highest record of the γ-decalactone production was 108.78 mg/L with 618.07% more than the wild type strain, which obtained from the fusant C3/14.

Słowa kluczowe

EN

Yarrowia lipolytica; protoplast fusion; gamma-decalactone

PL

Yarrowia lipolytica; łączenie protoplastów; gamma-dekalakton

• Wydawca: Wydawnictwo Politechniki Łódzkiej
• Czasopismo: Biotechnology and Food Science
• Rocznik: 2014
• Tom: Vol. 78, nr 1
• Strony: 3--13
• Opis fizyczny: Bibliogr. 25 poz.

Twórcy

autor

Gamil I.

• Flavours & Aroma Chemistry Department, National Research Center, Cairo, Egypt

autor

Khatteb A. E.-N.

• Genetics and Cytology Department, National Research Center, Dokki, Cairo, Egypt

autor

Shaaban M.

• Flavours & Aroma Chemistry Department, National Research Center, Cairo, Egypt

autor

Abd El-Mageed M.

• Flavours & Aroma Chemistry Department, National Research Center, Cairo, Egypt

autor

Talkhan F.

• Genetics and Cytology Department, National Research Center, Dokki, Cairo, Egypt

autor

Osman F.

• Flavours & Aroma Chemistry Department, National Research Center, Cairo, Egypt

Bibliografia

• 1. Endrizzi A, Pagot Y, Le CA, Nicaud JN, Belin JM. Production of lactones and peroxisomal beta-oxidation in yeasts. Crit Rev Biotechnol 1996, 16:301-329.
• 2. Gatfield IL. Biotechnological production of natural flavor materials. In: Flavor Chemistry: 30 Years of Progress ed. Teranishi R, Wick E, Hornstein I. 1999, pp. 211- 227. New York: Kluwer Academic, Plenum Publishers.
• 3. Schrader J, Etschmann M, Sell D, Hilmer J, Rabenhorst J. Applied biocatalysis for the synthesis of natural flavour compounds – current industrial processes and future prospects. Biotechnol Lett 2004, 26: 463-472.
• 4. Siek TJ, Albin IA, Sather LA, Lindsay RC. Comparison of flavor thresholds of aliphatic lactones with those of fatty acids, esters, aldehyde, alcohols and ketones. J Dairy Sci 1971, 54:1-9.
• 5. Mosandl A, Gunther C. Structure and properties of gamma-lactone enantiomers. J Agric Food Chem 1989, 37:413-418.
• 6. Lee SL, Cheng HY, Chen WC, Chou CC. Production of gamma-decalactone from ricinoleic acid by immobilized cells of Sporidiobolus salmonicolor. Process Biochem 1998, 33:453-459.
• 7. Wache Y, Aguedo M, Nicaud J-M, Belin J-M. Catabolism of hydroxyacids and production of lactones by the yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 2003, 61: 393-404.
• 8. Garcia E, Nicaud J-M, Belin J-M, Wache Y. Effect of acyl-CoA oxidase activity on the gamma-decalactone by the yeast Yarrowia lipolytica: A factorial approach. Biotechnol J 2007, 2: 1280-1285.
• 9. Wache Y, Aguedo M, Choquet A, beta-oxidation enzymes in the gamma-decalactones from methyl ricinoleate. Appl Environ Microbiol 2001, 67:5700-5704.
• 10. Fickers P, Fudalej F, Nicaud JM, Destain J, Thonart P. Selection of new over-producing derivatives for the improvement of extracellular lipase production by the non-conventional yeast Yarrowia lipolytica. J Biotechnol 2005, 115: 379-386.
• 11. Blin-Perrin C, Molle D, Dufosse L, Le-Quere J-L. Metabolism of ricinoleic acid into -beta-oxidation and long chain acyl intermediates of ricinoleic acid in the genus Sporidiobolus sp. FEMS Microbiol Lett 2000, 188: 69–74.
• 12. Dujon B, Sherman D, Fischer G, Durrens P. Genome evolution in yeasts. Nature 2004, 430: 35-44.
• 13. Aguedo M, Beney L, Wache Y, Belin JM. Mechanisms underlying the toxicity of lactone aroma compounds towards the producing yeast cells. J Appl Microbiol 2003, 94: 258-265.
• 14. Khattab A, Ibrahim G, Fatma N, Talkhan Osman F, Magda A. Abd El-Mageed El-Ghorab A Genetic improvement of the beta-oxidation pathway in the yeast Yarrowia lipolytica gamma-decalactone. Austr J Basic Appl Sci 2012, 6: 206-214.
• 15. Amaral PF, de-Almeida AP, Peixoto T, Rocha-Leao MH, Coutinho JA, Coelho MA. Beneficial effects of enhanced aeration using perfluorodecalin in Yarrowia lipolytica cultures for lipase production. World J Microbiol Biotechnol 2007, 23: 339-344.
• 16. Adelaide B, Gomes N, Belo I. Lipase induction in Yarrowia lipolytica for castor oil hydrolysis and its gamma-decalactone production. J Am Oil Chem Soc 2012, 89: 1041-1047.
• 17. Spencer TF, Laud P, Spencer D. The use of mitochondria mutants in the isolation of hybrid involving industrial yeast strain. II: Use in isolation of hybrid obtained by protoplast fusion. Mol GenGenet 1980, 179:651-654.
• 18. Farahanak F, Seki T, Ryu D, Ogrydziak D. Construction of lactose-assimilating and high ethanol-producing yeasts by protoplast fusion. Appl Environ Microbiol 1986, 51: 362-367.
• 19. Groguenin A. Wache Y. Escamilla, E. Aguedo M. Genetic engineering of the beta-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds. J Mol Catal B 2004, 28: 75-79.
• 20. Peberdy JF, Ferenczy L. Fungal protoplasts: Applications in biochemistry and genetics. 1985, Marcel Dekker, Inc.New York.Basel.
• 21. Lin C, Hsieh P, Mau J, Teng D. Construction of an inter-generic fusant from Schizosaccharomyces pombe and Lentnula edodes for xylan degradation and polyol production. Enzyme Micro Technol 2005, 36: 107-117.
• 22. Khattab A. Bazaraa. W. Screening, mutagenesis and protoplast fusion of Aspergillus niger for the enhancement of extracellular glucose oxidase production. J Ind Microbiol Biotechnol 2005, 32: 289-294.
• 23. Mansour A.F. Fatma N. Talkhan El-Ghorab A. Olive oil as a precursor for gamma-dodecalactone by biotransformation using Yarrowia lipolytica isolates. Bull Fac Agric, Cairo Univ, 2002, 53: 427-442.
• 24. Pagot Y, Endrizzi A, Nicaud JM, Belin JM. Utilization of an auxotrophic strain of the yeast Yarrowia lipolytica to improve gamma-decalactone production yields. Lett Appl Microbiol 1997, 25:113-116.
• 25. Cardillo R, Fuganti C, Sacerdote G, Barbeni M, Cabella P, Squarcia F. Procede de production microbiologique de decanolide gamma (R) et d’octanolide gamma (R). European Patent No. 1990, 0356291.