Morphological and genetic diversity of Isopyrum thalictroides L. (Ranunculaceae) populations of isolated forest fragments of different sizes in East Central Poland

• Autorzy: Skrajna T. , Kubicka H. , Ługowska M.

Identyfikatory

DOI

10.3161/15052249PJE2015.63.1.003

• Języki publikacji: EN

Abstrakty

EN

Isopyrum thalictroides is classified as an indicator species of the socalled ‘old-growth forests’. It occurs in shady deciduous forests and is a characteristic species of the order Fagetalia silvaticae. In many parts of Europe and Poland, it is classified in Red Lists of endangered species with different threat levels. Studies of an occurrence of I. thalictroides were carried out in the years 2000–2002 and 2010–2012 to examine changes in the abundance and morphological and genetic diversity of populations inhabiting small mid-field forest fragments (populations 2–4) and a compact forest (1 population), all located in east central Poland. Isopyrum thalictroides cover declined drastically at all isolated sites (from 83 to 93%) whereas the whole species diversity of these phytocenoses increased. By contrast, in the compact forest both, the area occupied by I. thalictroides population and the total community composition only slightly changed over time. When I. thalictroides plants sampled from all studied sites were compared in terms of the biometrical characteristics, populations 3 and 4 were found to be most similar, population 1 was less similar and population 2 was the most different of all populations, probably due to strong anthropogenic pressure. Electrophoretic analysis of seed and leaf storage proteins of Isopyrum thalictroides obtained from the four sites demonstrated that the populations were quite similar genetically, no matter how distant they were from each another. Population 2 was the most diverse, followed by population 1 whereas populations 3 and 4 were the most similar. A decreasing genetic distance in the I. thalictroides population would lead to inbreeding as seed setting by the plants will be poorer and the seeds will be less viable, which will result in a lower number of plants per a given area. The decreased gene pool indicates that the population of I. thalictroides is threatened with extinction in this area. Therefore, it is warranted to undertake some conservation measures to protect the species; hence the need arises to monitor it.

Słowa kluczowe

EN

biometric characteristics; polymorphism; storage proteins; SDS-PAGE method

PL

cecha biometryczna; polimorfizm; białka zapasowe; metoda SDS-PAGE

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2015
• Tom: Vol. 63, nr 1
• Strony: 23--37
• Opis fizyczny: Bibliogr. 41 poz., rys., tab., wykr.

Twórcy

autor

Skrajna T.

• Agricultural Ecology Department, University of Natural Sciences and Humanities, B. Prusa 14, 08-110 Siedlce, Poland

autor

Kubicka H.

• Polish Academy of Science, Botanical Garden Centre for Biological Diversity Conservation of Warsaw, Prawdziwka 2, 02-973 Warsaw, Poland

autor

Ługowska M.

• Agricultural Ecology Department, University of Natural Sciences and Humanities, B. Prusa 14, 08-110 Siedlce, Poland

Bibliografia

• 1. Baležentienė L. 2010 - Trilapė bligna (Isopyrum thalictroides L.) Kamšos draustinyje - Vytauto Didžiojo universiteto Botanikos sodo raštai, 14: 29-38 (in Lithuanian).
• 2. Broda B., Mowszowicz J. 2000 - Guide to determination of medicinal, poisonous and usable plants - Wyd. Lekarskie PZWL, Warszawa (in Polish).
• 3. Brunet J., Oheimb von G. 1998 - Migration of vascular plants to secondary woodlands in southern Sweden - J. Ecol. 86: 429-438.
• 4. Chassel D. 1977 - La description nom paramètrique de la dispersion spatiale des indyviduals d'une espece - Journèe francaise de biometrie, 28 april: 68.
• 5. Cieślak E., Paul W., Ronikier M. 2006 - Low genetic diversity in the endangered population of Viola uliginosa in its locus classicus at Rząstka near Cracow (Southern Poland) as revealed by AFLP markers - Acta Soc. Bot. Pol. 75: 245-251.
• 6. Čiuplys R. 2007 - Trilape bligna Isopyrum thalictroides L. iš: Lietuvos raudonoji knyga [Isopyrum thalictroides L. in Lithuanian Red Book] - Vilnius, 411 pp. (in Lithuanian).
• 7. Dzwonko Z. 2001 - Migration of vascular plant species to a recent wood adjoining ancient woodland - Acta Soc. Bot. Pol. 70: 71-77.
• 8. Dzwonko Z., Loster S. 2001 - Wskaźnikowe gatunki starych lasów i ich znaczenie dla ochrony przyrody i kartografii roślinności [Ancient woodland plant species indicators and their importance for nature conservation and vegetation mapping] - Prace Geog. 178: 119-132 (in Polish).
• 9. Dzwonko Z. 2007 - Przewodnik do badań fitosocjologicznych [Guidebook to phytosociological studies] - Vademecum geobotanicum, Sorus Poznań-Kraków, 304 pp. (in Polish).
• 10. Duarte J.M., Santos J.B., Melo L.C. 1999 - Comparison of similarity coefficients based on rapid markers in the common bean - Genetics and Molecular Biology, 22: 427-432.
• 11. Ellenberg H., Weber H., Dull R., Wirth V., Werner W., Paulissen D. 1992 - Zeigerverte von Pflanzen in Mitteleuropa - Scripta Geobot. 18: 1-258.
• 12. Ellstrand N.C., Elam D.R. 1993 - Population genetic consequences of small population size: implications for plant conservation - Annu. Rev. Ecol. Syst. 24: 217-243.
• 13. Falińska K. 1995 - Genet disintegration in Filipendula ulmaria: Consequences for population dynamics and vegetation succession - J. Ecol. 82: 865-877.
• 14. Gabrielsen T.M., Brochmann C. 1998 - Sex after all: high levels of diversity detected in the arctic clonal plant Saxifraga cernua using RAPD markers - Mol. Ecol. 7: 1701 -1708.
• 15. Geertsema W., Opdam P., Kropff M.J. 2002 - Plant strategies and agricultural landscapes: survival in spatially and temporally fragmented habitat - Landscape Ecol. 17: 263-279.
• 16. Głowacki Z., Falkowski M., Krechowski J., Marciniuk J., Marciniuk P., Nowicka-Falkowska K., Wierzba M. 2003 - Czerwona lista roślin naczyniowych Niziny Południowopodlaskiej [The red list of vascular plants of the Południowopodlaska Lowland] - Chrońmy Przyrodę Ojczystą, 59: 5-41 (in Polish).
• 17. Hoskovec L. 2007 - Isopyrum thalictroides L. - zapalice žluťuchovitá. http://botany.cz/cs/isopyrum-thalictroides/.
• 18. Hulten E., Fries M. 1986 - Atlas of North European vascular plants north of the Tropic of Cancer, vol. 2 - Koeltz Scientific Books. Federal Republic of Germany.
• 19. Istatkova R., Doncheva T., Philipov S. 2004 - A comparative study of the alkaloid composition of Isopyrum thalictroides (Ranunculaceae) - Phytology Balcanica, Sofia, 10: 257-259.
• 20. Jakubowska-Gabara J. 2001 - Endangered and threatened vascular plants of the forests of central Poland and the problems of their conservation - Nat. Conserv. 58: 43-56.
• 21. Jalas L., Suominen J. 1989 - Nymphaeaceae to Ranunculaceae. Atlas florae Europaea. Distribution of vascular plants in Europe 8: 39.
• 22. Käsermann Ch. 1999 - EN Isophyrum thalictroides L. - Muschelbliimchen - Rosaceae. https://www.infoflora.ch/assets/content/documents/merkblaetter_artenschutz_de/isop_thal_d.
• 23. Klimko K., Czarna A., Wysakowska I. 2000a -Stan obecny stanowisk Isopyrum thalictroides L. na terenie Wielkopolski. [Current status of Isopyrum thalictroides L. localities in the Wielkopolska] - Rocz. AR. Poz. CCCXXII, Bot. 3: 101-112 (in Polish).
• 24. Klimko K., Czarna A., Wysakowska I., Janyszek S. 2000b - Zdrojówka rutewkowata (Isopyrum thalictroides L.) w Wielkopolsce. Ekologia i zagrożenie. [Isopyrum thalictroides L. in the Wielkopolska. Ecology and threat] - Rocz. AR. Poz. CCCXXII, Bot. 3: 113-134 (in Polish).
• 25. Kondracki J. 2002 - Geografia regionalna Polski [The regional geography of Poland] - PWN Warszawa, 441 pp. (in Polish).
• 26. Kosiński I., Stefanowicz-Hajduk J., Ochocka R. 2009 - Demographic versus genetic (rapd) variation between and within two populations of the clonal plant Paris quadrifolia L. (Liliaceae) - Pol. J. Ecol. 57: 303-311.
• 27. Kubicka H., Carillo J.M., Benito C. 2005 - Zmienność morfologiczna i polimorfizm białek zapasowych w liniach wsobnych żyta ozimego (Secale cereale L.) [Morphological variability and polymorphism of storage proteins in inbred lines of winter rye (Secale cereale L.)] - Zesz. Nauk. Politechniki Białostockiej, Ekonomia i Zarządzanie, 10: 257-265 (in Polish).
• 28. MacArthur R.H., Wilson E.G. 1967 - The theory of island biogeography - Princeton Univ. Press, Princeton, New York: 1-203.
• 29. Matuszkiewicz W. 2001 - Przewodnik do oznaczania zbiorowisk roślinnych Polski [Guide-book to plant communities of Poland] - PWN, Warszawa, 537 pp. (in Polish).
• 30. Mirek Z., Piękoś-Mirkowa H., Zając A., Zając M. 2002 - Flowering plants and pteridophytes of Poland - a checklist (In: Biodiversity of Poland, Ed: Z. Mirek) - Wł. Szafer Institute of Botany, Polish Academy of Sciences, Kraków, 1,442pp.
• 31. Nowak A., Nowak S., Spałek K. 2008 - Red list of vascular plants of Opole Province 2008 - Opol. Scient. Soc.-Nature Journal, 41: 141-158.
• 32. Olaczek R. 1974 - Etapy pinetyzacji grądu [Phases of oak-linden-hornbeam forest pinetization] - Phytocoenosis, 3: 201-214 (in Polish).
• 33. Peterken G.F. 1974 - A method for assessing woodland flora for conservation using indicator species - Biol. Conserv. 6: 239-245.
• 34. Peterken G.F. 1977 - Habitat conservation priorities in British and European woodlands - Biol. Conserv. 1:223-236.
• 35. Rutkowski L. 2007 - Klucz do oznaczania roślin naczyniowych Polski niżowej [Key to identification of vascular plants in lowland Poland] - PWN Warszawa (in Polish).
• 36. Schmidt K., Jensen K. 2000 - Genetic structure and AFLP variation of remnant populations in the rare plant Pedicularis palustris (Scrophulariaceae) and its relation to population size and reproductive components - Am. J. Bot. 87: 678-689.
• 37. Skrajna T, Kubicka H., Rzymowska Z. 2012a - Phenotypic variation and polymorphism of storage proteins in the seeds of Bromus secalinus L. populations from the area of North-eastern Poland - Pol. J. Ecol. 60: 41-55.
• 38. Skrajna T., Kubicka H., Rzymowska Z. 2012b - Illecebrum vericillatum L. - endangered species in agrocenoses of eastern Poland: Assessment of ecological and genetic indicators for protection goals - Pol. J. Ecol. 60: 577-589.
• 39. Sobczyk M. 2007 - Statystyka [Statistics] - PWN Warszawa 428 pp. (in Polish).
• 40. Tutin T.G. 1964 - Isopyrum L. (In: Flora Europaea. 1: Lycopodiaceae to Plantaginaceae, Eds: T.G. Tutin, V.H. Heywood) - CUP, Cambridge 210.
• 41. Żukowski W., Jackowiak B. 1995 - Endangered and threatened vascular plants of Western Pomerania and Wielkopolska - Department of plant taxonomy, A. Mickiewicz Univ. in Poznań: 1-141.