Powiadomienia systemowe
- Sesja wygasła!
- Sesja wygasła!
- Sesja wygasła!
Tytuł artykułu
Treść / Zawartość
Pełne teksty:
Identyfikatory
DOI
Warianty tytułu
Języki publikacji
Abstrakty
Identification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea stagnalis) hepatopancreatic gland. Toward this end, mature individuals of pond snail (L. stagnalis) were treated with 50µM B[a]P solution in a short 36h static exposure test. Differential Display PCR (DD-PCR) was used to generate a unique cDNA fingerprint of genes that were differentially expressed in the tissues of exposed and unexposed snails. To assess the putative identity of the isolated cDNA amplicons (ESTs), BLAST queries were performed to find similarities in their nucleotide sequence. Real-Time qPCR analysis was used to verify the DD-PCR expression profile. Finally, an additional independent exposure study, including higher dose of B[a]P (100µM), was conducted to validate the expression of selected ESTs. BLAST revealed that only 3 out of 9 isolated ESTs had meaningful information on their putative nucleotide sequence identity. The highest similarity was scored for EST-A1, identified as the transcript of UAP-like protein, found to be up-regulated after B[a]P exposure. The original expression pattern that was observed in DD-PCR step was coherent with results of the qPCR verification for 3 out of 5 analyzed ESTs. However, changes in the ESTs expression were modest and the treatment with B[a]P resulted in significant down-regulation for only 1 unidentified fragment (EST-G42, almost 2-fold; p<0.05) when compared to untreated snails. Although no significant changes were observed for EST-A1 and EST-G42 in the validation study, their expression pattern was consistent with that obtained from DD-PCR. Surprisingly, EST-C5 remained in contrast to the DD-PCR part, but it showed significant down-regulation in group of snails exposed to 100µM B[a]P (3.5-fold; p<0.05). The obtained results show that diverse genes may be involved in the molecular response of the pond snail’s hepatopancreas to treatment with B[a]P. However, further research is needed to confirm the utility of the discovered EST as PAH biomarkers in biomonitoring practices with L. stagnalis as bioindicator species.
Czasopismo
Rocznik
Tom
Strony
8--17
Opis fizyczny
Bibliogr. 35 poz., rys., tab.
Twórcy
autor
- Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-709 Olsztyn, Poland; Phone: +48 89 5234151; Fax: +48 89 5234131
autor
- Department of Water and Waste-Water Technology, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, ul. Narutowicza 11/12, 80-233 Gdańsk, Poland
autor
- Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-709 Olsztyn, Poland
autor
- Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-709 Olsztyn, Poland
autor
- Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-709 Olsztyn, Poland
autor
- Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-709 Olsztyn, Poland
autor
- Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-709 Olsztyn, Poland
Bibliografia
- Altschul, S.F., T.L. Madden, A.A. Schäffer, J. Zhang, Z. Zhang, W. Miller, D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 17: 3389-3402.
- Arakane, Y., M. Baguinon, S. Jasrapuria, S. Chaudhari, A. Doyungan, K.J. Kramer, S. Muthukrishnan, R.W. Beeman. 2011. Two uridine-diphosphate N-acetylglucosamine pyrophosphorylases are critical for Tribolium castaneum molting, survival and fecundity. Insect Biochemistry and Molecular Biology 41: 42-50.
- Bailey, G.S., D.E. Williams, J.D. Hendricks. 1996. Fish models for environmental carcinogenesis: the rainbow trout. Environmental Health Perspectives 104: 5-21.
- Bandow, C., L. Weltje. 2012. Development of an embryo toxicity test with the pond snail Lymnaea stagnalis using the model substance tributyltin and common solvents. Science of the Total Environment 435-436: 90-95.
- Baumard, P., H. Budzinski, Q. Michon, P. Garrigues, T. Burgeot, J. Bellocq. 1997. Origin and bioavailability of PAHs in the Mediterranean Sea from mussel and sediment records. Coastal and Shelf Science 47: 77-90.
- Besnard, A.L., A. Bouétard, D. Azam, M.A. Coutellec. 2013. Isolation and characterization of three new multiplex sets of microsatellite markers in the hermaphroditic freshwater snail Lymnaea stagnalis (Mollusca, Gastropoda, Heterobranchia, Panpulmonata, Hygrophila) using 454-pyrosequencing technology. Molecular Ecology Resources. 13 p.
- Bouétard, A., A.L. Besnard, A.L.D. Vassaux, L. Lagadic, M.A. Coutellec. 2013. Impact of the redox-cycling herbicide diquat on transcript expression and antioxidant enzymatic activities of the freshwater snail Lymnaea stagnalis. Aquatic Toxicology 126: 256-265.
- Brzuzan P., L. Jurczyk, T. Foks. 2007. Differential gene expression in whitefish (Coregonus lavaretus) exposed to benzo pyrene. In: Biology and Management of Coregonid Fishes-2005 (ed. M. Jankun, P. Brzuzan, P. Hliwa, M. Luczynski). Archiv für Hydrobiologie, Special Issues: Advances in Limnology 60: 147-155.
- Byzitter, J., K. Lukowiak, V. Karnik, S. Dalesman. 2012. Acute combined exposure to heavy metals (Zn, Cd) blocks memory formation in a freshwater snail. Ecotoxicology 21: 860-868.
- Choy, E.J., Q. Jo, H.-B. Moon, C.-K. Kang, J.-C. Kang. 2007. Time-course uptake and elimination of benzo(a)pyrene and its damage to reproduction and ensuing reproductive outputs of Pacific oyster, Crassostrea gigas. Marine Biology 151: 157-165.
- Corton, J.J. 1998. Differential Display PCR. In: PCR Protocols in Molecular Toxicology (ed. J.P. Vanden Heuvel), pp. 99-120. CRC Press, New York.
- Feng, Z.P., Z. Zhang, R.E. Van Kesteren, V.A. Straub, P. Van Nierop, K. Jin, N. Nejatbakhsh, J.I. Goldberg, G.E. Spencer, M.S. Yeoman, W. Wildering, J.R. Coorssen, R.P. Croll, L.T. Buck, N.I. Syed, A.B. Smit. 2009. Transcriptome analysis of the central nervous system of the mollusc Lymnaea stagnalis. BMC Genomics 10: 1-15.
- Giusti, A., A. Barsi, M. Dugué, M. Collinet, J.P. Thomé, J. Joaquim-Justo, B. Roig, L. Lagadic, V. Ducrot. 2013. Reproductive impacts of Tributyltin (TBT) and Triphenyltin (TPT) in the hermaphroditic freshwater gastropod Lymnaea stagnalis. Environmental Toxicology and Chemistry 7: 1552-1560.
- Gust, M., M. Fortier, J. Garric, M. Fournier, F. Gagne. 2013. Effects of short-term exposure to environmentally relevant concentrations of different pharmaceutical mixtures on the immune response of the pond snail Lymnaea stagnalis. Science of the Total Environment 445-446: 210-218.
- Leonard, E.M., C.M. Wood. 2013. Acute toxicity, critical body residues, Michaelis–Menten analysis of bioaccumulation, and ionoregulatory disturbance in response to waterborne nickel in four invertebrates: Chironomus riparius, Lymnaea stagnalis, Lumbriculus variegatus and Daphnia pulex. Comparative Biochemistry and Physiology 158: 10-21.
- Ma, P., J. Winderickx, D. Nauwelaers, F. Dumortier, A. de Doncker, J.M. Thevelein, P. van Dijck. 1999. Deletion of SFI1, a novel suppressor of partial Ras-cAMP pathway deficiency in the yeast Saccharomyces cerevisiae, causes G2 arrest. Yeast 15: 1097-1109.
- Maliszewska-Kordybach, B. 1999. Sources, concentrations, fate and effects of polycyclic aromatic hydrocarbons (PAHs) in the environment. Part A: PAHs in air. Polish Journal of Environmental Studies 8: 131-136.
- Martinez-Sanz, J., A. Yang, Y. Blouquit, P. Duchambon, L. Assairi, T. Craescu. 2006. Binding of human centrin 2 to the centrosomal protein hSfi1. FEBS Journal 273: 4504-4515.
- Merzendorfer, H., H. Shin Kim, S.S. Chaudhari, M. Kumari, C.A. Specht, S. Butcher, S.J. Brown, J.R. Manak, E.W. Beeman, K.J. Kramer, S. Muthukrishnan. 2012. Genomic and proteomic studies on the effects of the insect growth regulator diflubenzuron in the model beetle species Tribolium castaneum. Insect Biochemistry and Molecular Biology 42: 264-276.
- Miller, K.P., K.S. Ramos. 2001. Impact of cellular metabolism on the biological effects of benzo pyrene and related hydrocarbons. Drug Metabolism Reviews 33: 1-35.
- Munley, K.M., K.V. Brix, J. Panlilio, D.K. Deforest, M. Grosell. 2013. Growth inhibition in early life-stage tests predicts full life-cycle toxicity effects of lead in the freshwater pulmonate snail, Lymnaea stagnalis. Aquatic Toxicology 128: 60-66.
- Nesto, N., S. Romano, V. Moschino, M. Mauri, L. Da Ros. 2007. Bioaccumulation and biomarker responses of trace metals and micro-organic pollutants in mussels and fish from the Lagoon of Venice, Italy. Marine Pollution Bulletin 55: 469-484.
- Ng, T.Y.T., N.M. Pais, C.M. Wood. 2011. Mechanisms of waterborne Cu toxicity to the pond snail Lymnaea stagnalis: Physiology and Cu bioavailability. Ecotoxicology and Environmental Safety 74: 1471-1479.
- Peters, W. 1972. Occurrence of chitin in Mollusca. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 41: 541-544.
- Pfaffl, M.W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 29: 2002-2007.
- Pfaffl, M.W., G.W. Horgan, L. Dempfle. 2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 30: 1-10.
- Prasch, A.L., H. Teraoka, S.A. Carney, W. Dong, T. Hiraga, J.J. Stegeman, W. Heideman, R.E. Peterson. 2003. Aryl hydrocarbon receptor 2 mediates 2,3,7,8-tetrachlorodibenzo-p-dioxin developmental toxicity in zebrafish. Toxicological Sciences 76: 138-150.
- Sadamoto, H., H. Takahashi, T. Okada, H. Kenmoku, M. Toyota, Y. Asakawa. 2012. De novo sequencing and transcriptome analysis of the central nervous system of mollusc Lymnaea stagnalis by deep RNA sequencing. PloS one 7: 1-13.
- Sokolov, B.P., D.J. Prockop. 1994. A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes. Nucleic Acids Research 22: 4009-4015.
- Teraoka, H., W. Dong, Y. Tsujimoto, H. Iwasa, D. Endoh, N. Ueno, J.J. Stegeman, R.E. Peterson, T. Hiraga. 2003. Induction of cytochrome P450 1A is required for circulation failure and edema by 2,3,7,8-tetrachlorodibenzo-p-dioxin in zebrafish. Biochemical and Biophysical Research Communications 304: 223-228.
- Thiele, S., G.W. Brummer. 2002. Bioformation of polycyclic aromatic hydrocarbons in soil under oxygen deficient conditions. Soil Biology & Biochemistry 34: 733-735.
- USEPA. 1982. Office of the Federal Registration (OFR), Appendix A: Priority pollutants. Federal Register 47: 52309.
- Wang-Gillam, A., I. Pastuszak, A.D. Elbein. 1998. A 17-amino acid insert changes UDP-N-acetylhexosamine pyrophosphorylase specificity from UDP-GalNAc to UDP-GlcNAc. The Journal of Biological Chemistry 273: 27055-27057.
- Westerfield, M. 2000. The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio). 4th ed. University of Oregon Press, Eugene.
- Woźny, M., P. Brzuzan, L. Wolińska, M. Góra, M.K. Łuczyński. 2012. Differential gene expression in rainbow trout (Oncorhynchus mykiss) liver and ovary after exposure to zearalenone. Comparative Biochemistry and Physiology, Part C 156: 221-228.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-f318759a-14cb-4c46-9e10-7feff4e3ae59