The membrane with polylactide and hyaluronic fibers for skin substitute

• Autorzy: Stodolak-Zych E. , Rozmus K. , Dzierzkowska E. , Zych Ł. , Kapacz-Kmita A. , Gargas M. , Kołaczkowska E. , Cieniawska M. , Książek A. , Ścisłowska-Czarnecka A.

Identyfikatory

DOI

10.5277/ABB-01199-2018-02

• Języki publikacji: EN

Abstrakty

EN

Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells. We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation. Methods: Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions. By changing the parameters, it was possible to control the diameter and properties of both polymer fibers. The spinning solution were characterized by surface tension and rheology. A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA. Structure of the PLA/HA nonwoven was analyzed using spectroscopy (FTIR/ATR). Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions. Results: The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt. solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C. The hyaluronic fibers were prepared from a 12% wt. solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol. All materials were biocompatible but to a different degree. Conclusions: The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers. The material positively underwent biocompatibility testing in contact with fibroblasts.

Słowa kluczowe

EN

nanofibrous scaffold; electrospinning; skin substitute; hyaluronic acid; polylactide

PL

nanowłókna; elektroprzędzenie; kwas hialuronowy; polilaktyd

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2018
• Tom: Vol. 20, no. 4
• Strony: 91--99
• Opis fizyczny: Bibliogr. 25 poz., rys., tab., wykr.

Twórcy

autor

Stodolak-Zych E.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Krakow, Poland

autor

Rozmus K.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Krakow, Poland

autor

Dzierzkowska E.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Krakow, Poland

autor

Zych Ł.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Krakow, Poland

autor

Kapacz-Kmita A.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Krakow, Poland

autor

Gargas M.

• The Jagiellonian University, Department of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Krakow, Poland

autor

Kołaczkowska E.

• The Jagiellonian University, Department of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Krakow, Poland

autor

Cieniawska M.

• Academy of Physical Education, Department of Physiotherapy, Section of Anatomy, Krakow, Poland

autor

Książek A.

• Academy of Physical Education, Department of Physiotherapy, Section of Anatomy, Krakow, Poland

autor

Ścisłowska-Czarnecka A.

• Academy of Physical Education, Department of Physiotherapy, Section of Anatomy, Krakow, Poland

Bibliografia

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Uwagi

This study was performed within the framework of funding for statutory activities of AGH University of Science and Technology in Krakow, Faculty of Materials Science and Ceramics (11.11.160.182). The biological studies were co-funded by the National Science Centre of Poland (grant No. 2014/15/B/NZ6/02519).