The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films

• Autorzy: Parizek M. , Base T. , Londesborough M. G. S. , Lisa V. , Bacakova L.
• Języki publikacji: EN

Abstrakty

EN

Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2 B10H11(A), 1,2-(HS)2-1,2-C2B10H10(B), 9,12-(HS)2-1,2-C2B10H10(C) and 1,12-(HS)2-1,12- C2B10H10(D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.

Słowa kluczowe

EN

metal coatings; gold film; carboranethiol; cell adhesion; cell spreading; cell proliferation; biomaterials; tissue engineering; surgical implants

PL

implanty chirurgiczne; biomateriały; powłoki metalowe; inżynieria materiałowa

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2008
• Tom: Vol. 11, no. 75
• Strony: 6--8
• Opis fizyczny: Bibliogr. 9 poz., wykr., zdj.

Twórcy

autor

Parizek M.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Base T.

• Institute of Inorganic Chemistry, Acad. Sci. CR, 250 68 Husinec-Rez 101, Czech Republic

autor

Londesborough M. G. S.

• Institute of Inorganic Chemistry, Acad. Sci. CR, 250 68 Husinec-Rez 101, Czech Republic

autor

Lisa V.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Bacakova L.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

Bibliografia

• [1] Bacakova L., Mares V., Bottone M. G., Pellicciari C., Lisa V., Svorcik V.: J. Biomed. Mater. Res. 49: 369-379, 2000.
• [2] Bacakova L., Walachova K., Svorcik V., Hnatowitz V.: J. Biomater. Sci. Polymer Edn., 12: 817-834, 2001.
• [3] Bacakova L, Filova E, Rypacek F, Svorcik V, Stary V: Physiol. Res. 53 Supp: S35-S45, 2004.
• [4] Base T., Bastl Z., Plzak Z., Grygar T., Plesek J., Carr M.J., Malina V., Subrt J., Bohacek J., Vecernikova E., Kriz O.: Langmuir 21: 7776-7785, 2005.
• [5] McGhee A.M., Procter D.J.: Top. Curr. Chem. 264: 93, 2006.
• [6] Rautaray D., Sastry M.: Biotechnol. Prog. 21: 1759-1756, 2005.
• [7] Berry C.C., de la Fuente J.M., Mullin M., Chu S.W., Curtis A.S.: IEEE Trans Nanobioscience 6: 262-269, 2007.
• [8] de la Fuente J.M., Alcantara D., Penades S.: IEEE Trans Nano-bioscience 6: 275-281, 2007.
• [9] Park S.Y., Lytton-Jean A.K., Lee B., Weigand S., Schatz G.C., Mirkin C.A.: Nature 451: 553-556, 2008.